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العنوان
Host- Gut Microbiota Relationship
in Atopic Bronchial Asthma /
المؤلف
Amin, Doaa Abdelrazek Mohamed.
هيئة الاعداد
باحث / Doaa Abdelrazek Mohamed Amin
مشرف / Ashraf Mahmoud Okba
مشرف / Asmaa Saber Moustafa
مناقش / Mariam Maged Amin
تاريخ النشر
2015.
عدد الصفحات
182p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الطب الباطني
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة عين شمس - كلية الطب - باطنة عامة
الفهرس
Only 14 pages are availabe for public view

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Abstract

Summary
Bronchial Asthma is a disease with multifactor
etiology (Ito et al., 2006). It is defined by the global and
initiative for asthma management and prevention GINA as
chronic inflammatory disorder affecting the airways, in
which many cells and cellular elements play a role. The
chronic inflammation is associated with airway hyperresponsiveness
that leads to recurrent episodes of
wheezing, breathlessness, chest tightness particularly at
night or in the early morning (GINA, 2012).
Numerous studies have addressed the composition of
the microbiota in healthy and allergic subjects. Gut
microbiota is an assortment of microorganisms inhabiting
the length and width of mammalian GIT. It is estimated that
human microbiota contains as many as 1014 bacterial cells,
a number that is 10 times greater than the number of human
cells present in our bodies. There are more than 3 millions
of microbial genes in our gut microbiota, 150 times more
than human genome (Duncan et al., 2007).
Increasing interest in host-microbiota relationship
and its effects on the immune system has led to studies
investigating the role of the microbiome in susceptibility to
diseases (Markle et al., 2013). According to Tanaka et al.
studies suggested that perturbations in the gastrointestinal
microbiota as a result of change in diet and antibiotic use
result in underdeveloped microbiota. This immature
microbiota delays proper maturation of the immune system,
disrupting the normal sequence of events that promote the
development of immunological tolerance increasing the
incidence of allergic hypersensitivity (Tanaka et al., 2009).
Aim of work was to study the variability of gut
microbiota in healthy and asthmatic patients and investigate
its diversity according to gender.
This study was a case control study conducted on
two groups 80 adult asthmatic patients and 40 healthy
individuals. Each group was divided into further subgroups
(females and males) to assess the variability of gut
microbiota by conventional stool culture in relation to
bronchial asthma. Data was collected regarding gender,
age, clinical history and specific investigations as (skin test
and pulmonary function testing). patient <18 years old, and
>45 years old, patients with old history of active infectious
conditions within the previous 30 days, autoimmune
diseases, recent diarrhea or constipation, on prebiotics or
probiotics, on corticosteroids for the past 6 weeks, on
immunotherapy or on regular diet free of any fermented
milk products for the last month, obese patients and
consumption of antibiotics within 30 days prior to sample
collection were excluded.
Results clearly showed that as regard age, duration of
illness, skin test results and pulmonary function test results,
no significant difference between males and females in
atopic patients was found. As regards diversity of gut
microbiota between the two studied groups results of
conventional stool culture showed no statistical significant
difference. When comparing between both groups
lactobacilli was found more in male patients compared to
healthy male group and it was of statistical significant
value. There was no correlation between number of
organisms and disease related parameters including age,
duration of illness, number of allergens and pulmonary
function test. To the best of our knowledge, no studies were
found on microbiota diversity in adults patients
The limitation of this study was that is was confined
to specific population related to a certain environment.
Also the studied group was done a small scale. Stool
culture couldn‘t detect all required organisms