الفهرس 
General IntroductionOnly 14 pages are availabe for public view
 |  | from | 274 |  |  |
| | | from | 274 | | |
Abstract
This part involves an introduction about coccidiosis, causative parasites, disease symptoms, negative impact on poultry, classification and mode of action of anticoccidial drugs
Part II: Literature review
This part includes a review of the physical and chemical properties of the studied anticoccidial drugs, Amprolium hydrochloride and Ethopabate. Also, it includes different analytical methods reported in the literature that aimed for the determination of the selected drugs in pure form, mixtures, pharmaceutical formulation, and biological media.
Part III: Spectrophotometric determinations of some anticoccidial drugs
This part is divided into six sections:
Section A: Simultaneous determination of Amprolium hydrochloride and Ethopabate by derivative ratio spectrophotometric method
The first derivative of the ratio spectra for each of Amprolium hydrochloride and Ethopabate is obtained. Amprolium hydrochloride is measured at 234.7 nm and Ethopabate is measured at 306.8 nm with mean recovery percentages of 99.76 ± 0.907 and 100.29 ± 0.847, respectively.Section B: Simultaneous determination of Amprolium hydrochloride and Ethopabate by mean centering spectrophotometric method
The obtained ratio spectra are mean centered and the peak amplitude is measured at 238.8 nm for Amprolium hydrochloride and 313 nm for Ethopabate with mean recovery percentages of 100.26 ± 1.018 and 99.93 ± 1.281, respectively.
Section C: Simultaneous determination of Amprolium hydrochloride and Ethopabate by ratio difference spectrophotometric method
For the determination of Amprolium hydrochloride in presence of Ethopabate, the amplitude difference of the ratio spectra at 239 and 310 nm is measured with mean recovery percentage of 99.27 ± 0.892. For the determination of Ethopabate in presence of Amprolium hydrochloride, the amplitude difference of the ratio spectra at 239 and 313 nm is measured with mean recovery percentage of 100.40 ± 1.814.
Section D: Simultaneous determination of Amprolium hydrochloride and Ethopabate by dual wavelength spectrophotometric method
Different sets of wavelengths are tried. Absorbance values of ETH are the same at 235.3 and 308 nm therefore these two wavelengths are selected for determination of AMP with mean recovery percentage of 99.30 ± 1.097. The same for the two wavelengths 244 and 268.4 nm at which the absorbance values of AMP are the same, hence these two wavelengths are selected for determination of ETH with mean recovery percentage of 100.02 ± 1.061.
Section E: Simultaneous determination of Amprolium hydrochloride and Ethopabate by area under the curve spectrophotometric method
Area under curve of the absorption spectra in the wavelength ranges 235.6-243 nm (λ1 - λ2) and 268.3–275 nm (λ3 – λ4) for each of AMP and ETH is calculated. The concentrations of AMP and ETH can be obtained by applying Cramer’s rule with mean recovery percentages of 100.38 ± 1.006 and 100.39 ± 0.955, respectively.