الفهرس 
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Abstract
1-studies the comparative immunizing power of binaryethylenimine (bei) and formalin inactivated blackleg cultures showed that ci chauvoei culture was completely inactivated 2 and 6 days after addition of bet in concentration of 0.1 and 0.05% respectively. While formalin inactivated the same culture after 15 days in concentration of 0.5%.
Moreover the protection percent in vaccinated guinea pigs were 100% for the two concentration of bei and 80% for cl chauvoei culture inactivated by using 0.5%
Formalin.
2-studies the comparative immunizing power of bei and formalin inactivated haemorrhagic septicaemia culture revealed that bel was completely inactivated past. Multocida. Organisms after 18 and 48 hours by using 0.1% and 0.05% bei respectively, while the same culture was inactivated 48 hours after addition of 0.5% formlin. The protection rate was 100% for the two concentration of bei and 80% after using 0.5 formalin.
3-the protective power of blackleg vaccines (monovalent aluminium hydroxide gel, monovalent oil adjuvant, multicomponent aluminium hydroxide gel, and multicomponent oil adjuvant) were (100%, 100%, 80%), (100%, 100%, 100%), (100%, 80%, 60%) and (100%, 100%, 80%) respectively in guinea pigs when challenged by 32, 64 and 96 mld of cl. Chauvoei spore suspension.
4-the agglutinating titre against blackleg vaccines in sera of guinea pigs were 0.1 it, 0.036 al, 0.28 al and 0.094 al respectively for monovalent aluminium hydroxide gel, monovalent oil adjuvant, multicomponent aluminium hydroxide gel and multicomponent oil adjuvant vaccines.
The agglutinating titres of blackleg vaccines in sera of calves were determined from the first month (0.11 al, 0.04 al, 0.12 al and 0.08 al) for monovalent aluminium hydroxide gel, monovalent oil adujvant, multicomponent aluminium hydroxide gel and mulitcomponent oil adjuvant vaccines respectively. Two months post vaccination, the titre reached its peak (0.064, 0.007, 0.08 and 0.042 al). from 3 months on ward there was a gradual decline in the titre which was more rapide in animals vaccinated with aluminium hydroxide gel vaccines than those vaccinated with oil adjuvant vaccines which remianed at the protective level till 10,11 months (0.5/il and 0.5pl) post vaccination for multicomponent and monovalent oil adjurent vaccines respectively. When elisa test was done on the same sera samples obtained from vaccinated cattle, the results run parallel with that of agglutination test except that the animls were considered in the protective level for 12 month after vaccination with mononovalent and multicomponent oil adjuvant vaccines (1.8 and 1.16 elisa unit respectively). While the titre in animlas vaccinated with monovalent and multiconpoent aluminium hydroxide gel vaccines considered within the protective level till 8,7 months (1.0, 1.1 elisa
Unit) respectively. Evaluation of h.s. Vaccines in cattle, indirect haemagglutination test as well as elisa test were applied on sera of vaccinated cattle. The results reveled that the haemagelutinating titre was raised at the begining of the first month and reached its peak 2 months post vaccination. The titre remained within the protective levcel for 6 months after using monovalent or multicomponent aluminium hydroxide gel vaccines and for 11 months post vactination with monovalent or multicomponent oil adjuvant vaccines. The serum neutraliztion test on sera of cattle vaccinated with different r.v.f. And fmd vaccines revealed that the superiority of oil adjuvant vaceines either monovalent or multicomponent as the titre still within the protective level till 11, 10 month post vaccination with monovalent and multicomponent rvf oil adjuvant vaccines respectively, and till 11 months for both monovalent and inulitcomponent fmd oil adjuvant vaccines.