الفهرس 
Introduction and Review of LiteratureOnly 14 pages are availabe for public view
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Abstract
The present work was performed to study the possible
protective role of green tea and curcumin as an antioxidants and
free radical scavengers against changes induced by 2, 3, 7, 8-
Tetrachlorodibenzo-p-dioxin (TCDD) administration to male Swiss
albino mice. In this study, serum protein fraction, differential white
blood cells, AhR level, CYP450 level, P53 level, CAT activity,
SOD activity and GSH concentration as well as body weight level
and the histological changes in the liver, kidneys, spleen and testes
were studied.
The present work included two experiments:
Experiment I
The mice were randomly divided into seven groups, 20 mice for
each:
· The first group: served as a control where mice were given
oral dose of vehicles only.
· The second group: served as a control where mice were
given oral dose with tween-80 only.
· The third group: served as a control where mice were
given oral dose with corn oil only.
· The fourth group: designated TCDD, where mice were
given oral dose of TCDD (1.332 mg/kg b.w).
· The fifth group: designated GT and TCDD, where mice
were given oral dose of green tea (0.5mg/kg b.w) 30 min
before the administration TCDD.
· The sixth group: designated Cur. and TCDD, where mice
were given oral dose of curcumin (0.5mg/kg b.w) 30 min
before the administration TCDD.
· The seventh group: designated of combined dose of GT,
Cur. + TCDD, where mice were given oral dose of green
tea & curcumin (1 mg/kg b.w) 30 min before the
administration TCDD.
All the above doses were repeated daily for 30 days.
Experiment II
Eighty mice were given daily oral doses of TCDD (1.332 mg/kg
b.w). After 30 days of TCDD administration, the mice were
divided into four subgroups, 20 mice each.
· The first subgroup: designated (Recov), was left for
recovery without any type of treatment for 30 days.
· The second subgroup: designated (Recov + GT), where
mice were given daily oral dose of green tea (0.5 mg/kg
b.w) for 30 days.
· The third subgroup: designated (Recov + Cur.) where
mice were given daily oral dose of curcumin (0.5 mg/kg
b.w) for 30 days.
· The fourth subgroup: designated (Recov +GT+ Cur.)
where mice were given combined oral dose of green tea &
curcumin daily (1 mg/kg b.w) for 30 days.
The main results obtained from this study are summarized as
the following:
· TCDD administration greatly increased AhR level, CYP450 level
in the liver homogenates versus those of control animals.
· TCDD administration greatly decreased P53 level in the liver
homogenates versus those of control animals.
· TCDD administration greatly decreased the levels of IgG light &
heavy chain, antitrypsin and albumin of blood serum in different
treated mice versus those of control animals.· TCDD administration greatly increased transferrin, haptoglopin
and acid glycoprotein levels of blood serum in different treated
mice versus those of control animals.
· TCDD administration greatly decreased total white blood cells
count (WBC); lymphocytes, eosinophils, monocytes and
basophiles levels where it increased level of neutrophils of blood
serum in different treated mice versus those of control animals.
· TCDD administration greatly inhibited CAT, SOD activites and
GSH concentration in the liver, kidneys, spleen and testes
homogenates versus those of control animals.
· TCDD administration greatly decreased body weight in different
treated animals versus those of control animals.
· Green tea & curcumin exerted a powerful inhibitory effect on the
the levels of AhR and CYP450 in the examined tissues compared
to those subjected to TCDD administration.
· Green tea & curcumin exerted a powerful stimulatory effect on
the level of P53 in the examined tissues compared to those
subjected to TCDD administration.
· Green tea & curcumin exerted a powerful inhibitory effect on the
increased levels of transferrin, haptoglopin and acid glycoprotein
levels of blood serum compared to those subjected to TCDD
administration.
· Green tea & curcumin exerted a powerful stimulatory effect on
the levels of IgG light & heavy chain, antitrypsin and albumin of
blood serum compared to those subjected to TCDD
administration.
· Green tea & curcumin exerted a powerful stimulatory effect on
the level of total white blood cells count (WBC); lymphocytes,
eosinophils, monocytes and basophiles levels and they were found to decrease the level of neutrophils of blood serum
compared to those subjected to TCDD administration.
· Green tea & curcumin exerted a powerful stimulatory effect on
the activities of CAT and SOD as well as restoration of the
concentration of GSH in the examined tissues compared to those
subjected to TCDD administration.
· Green tea & curcumin exerted a powerful stimulatory effect
on the level of body weight in different treated animals compared
to those subjected to TCDD administration.
· The combined doses of (GT+Cur.) were found to increase the
levels of P53, IgG light & heavy chain, transferrin, albumin,
total white blood cells count (WBC); lymphocyts, eosinophils,
monocytes, basophiles levels; activity of CAT, SOD and GSH
concentration in TCDD-exposed mice of experiment I and II
compared with curcumin.
· TCDD-administration markedly induced severe histological
changes in the liver, kidney, spleen and testes as well as the fine
structures of the liver compared with control animals.
· TCDD-administration markedly increased the amount of
collagenous fibers in the liver, kidney, spleen and testes versus to
those of control mice.
· TCDD-administration reduced the amount of protein in the liver
and kidney versus to those of control mice.
· TCDD-administration reduced the amount of carbohydrate
materials in the hepatic, renal and splenic tissues versus the
contents of control animals.
· Green tea & curcumin proved to have strong protective effect in
the prevention of TCDD-induced toxicity and malfunctions in the
studied organs.· Green tea & curcumin administration reduced the amount of
collagenous fibers in the studied organs.
· Green tea & curcumin administration restored the amount of
protein in the hepatic and renal tissues.
· Green tea & curcumin administration restored the amount of
carbohydrate material in liver, kidney and spleen.
· The combined dose of (GT+Cur.) were found to have strong
protective effect in the prevention of TCDD-induced toxicity; it
was found that they reduced the amount of collagenous fibers,
restored the amount of protein in liver and kidney and also
restored the amount of carbohydrate material in liver, kidney
and spleen.
· Comparison between the recovery of the mice after TCDD
administration by lifting the animals without any treatment or
by giving them Green tea & curcumin. The results showed that
green tea & curcumin significantly enhanced the recovery rat of
mice.
· It was found that the green tea & the combined dose of
(GT+Cur.) exhibited more protective effect than curcumin.
· It could be concluded that the protective role of green tea and
curcumin against TCDD toxicity is clear in experiment I
(protective) and experiment II (recovery). However, the
protective role of green tea and curcumin against toxicity of
TCDD in experiment I was more potent and more powerfull
than that of experiment II (recovery).