الفهرس 
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Abstract
Yet, the incidence of Equine herpes viruses in Egypt is still unclear and needs more investigation. This initiated the present design of a protocol for a rapid detection and characterization of Equine herpes virus from field specimens. A total of 93 samples were collected from governmental and private studs of Arabian horses’ including; 73 nasal swabs, 15 uterine swabs, and 5 aborted foeti and/or still birth tissues. Serologically, Dot and cell ELISAs were performed on 87 samples using polyclonal antibodies against type-1, -2, and -4 EHVs. 44.82% were clearly strong positive when detected by Dot ELISA. On the other hand 65 samples were positive when tested by cell ELISA. Characterization and typing of equine herpes viruses by seminested PCR was performed only on 28 selected samples from the highly distinct positive ones by both dot and cell ELISA representing all genotypes, in addition to, sex uterine samples not previously tested by ELISA. Specific primers for gB gene of EHV-2 and EHV-4 and gH gene of EHV-1 were used in the amplification reactions. 4 samples had been positive by ELISA were found negative by PCR.17 from 25 positive samples tested were found EHV-2, while 9 from 13 positive samples were typed EHV-4 and 4 from 18 positive samples were typified EHV-1. Sequencing of EHV-1 and EHV-2 samples gives 94-95% and 89-96 % homology, respectively.