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Abstract
The main objective of the present study was to isolate and identify the most common bacterial causes of mortalities and low productivity among cultured fish and assessed the value of using multiplex PCR in rapid detection of bacterial fish pathogens. This investigation was carried out in Abbassa Central Laboratory of Aquaculture Research, Sharkia Governorate, Egypt. A total number of 150 clinically and grossly diseased Nile tilapia (Oreochromis niloticus) were collected from different fish farms and transferred alive or recently dead and subjected to full clinical, postmortem and bacteriological examinations.
The results of this study could be summarized as follows:
1- Naturally infected O. niloticus showed that the most common clinical signs noticed due to bacterial pathogens infections were decrease of feed intake, swam near the surface of water, increased mucus secretion and congested gills. Hemorrhages at the base of fins, abdominal part, gill cover and necrotic area on the gill cover were also noticed. Abdominal distention and intestinal prolepses were observed. .