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Abstract
6- Respiratory disease conditions are one of the most serious groups of diseases affecting poultry. Respiratory manifestations commonly accompanied with mortality and decreased body weight and egg production.
Pasteurellosis and Ornithobacteriosis cause serious economic losses among chicken.
This study aimed to:Isolation
and identification of ORT from broiler chicken using conventional
laboratory methods, isolation and identification of Psteurella species from broiler chicken, application of PCR for direct detection of ORT DNA in nasal and tracheal swabs as well as lungs tissues collected from broiler chickens, application of PCR for confirmation the identification of biochemically identified ORT isolates, in vitro-antibiotic sensitivity test of the isolated ORT and Pasteurella to choose the most effective antibiotic for treatment.
This study was carried-out on Nasal and tracheal swabs as well as lungs and air sacs that collected from broiler chickens at Kafr El-Sheikh and Behera Governorates. Three hundreds and four chicken sample of different ages.
The samples were cultured into nutrient broth and incubated at 37 °C for 6 hrs. A loopfull from incubated culture broth was streaked onto sheep blood agar media with and without antibiotics (Polymmxin and Gentamycin).
The inoculated plates were incubated at 37 °C for 48 hrs under aerobic conditions and at 7.5 % CO2.
The results obtained can be summarized in:
Isolation of ORT from chickens showing respiratory manifestations:ORT
could not be isolated from nasal swabs collected from chickens showing
respiratory manifestations.
ORT could be isolated from 3.62 % examined tracheal swabs collected from chickens showing respiratory manifestation and could be isolated from air sacs and lungs of chickens showing respiratory manifestation at a percentage of 6.9 %.
The results of application of PCR for detection and identification of ORT:All ORT isolates (21 isolates ) were positive for PCR.
PCR could detect ORT in all samples from which the organism has been isolated. One out of 10 examined tracheal swabs from which the organism could not be
isolated gave a positive result with PCR (10 %). Moreover 2 out of 10 examined lungs and air sac from which the organism could not be isolated gave a positive results with PCR (20
%).
Results of isolation of Pasteurella multocida from chicken showing respiratory manifestations:The
results indicated that Pasteurella multocida was isolated from 4.60 % examined
samples.
The results also, indicated that ORT was isolated alone from 13 samples , Pasteurella multocida alone from 4 samples and both ORT and Pasteurella multocida from 10 samples.
The antibiotic sensitivity of ORT and Pasteurella multocida isolated from chickens suffering from respiratory manifestations revealed that most of ORT isolates were sensitive to Amoxicillin, Choloramphenicol, Erythromycin and Lincospectin, and most Pasteurella multocida isolated from chickens showing respiratory manifestations were sensitive to Amoxicilline, Chloramphenicol, Erythromycin and Lincospectin.