الفهرس 
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Abstract
MRSA is an important nosocomial pathogen and its prevalence !nas increased world wide in recent years. The continuos resistance to newly applied antibiotics became one of the most common features of MRSA. In addition, MRSA is also an expensive problem, for hospitals, the average cost for treating a patient with MRSA infection is approximately seven times more than that of the same patient with MSSA infection. Preventing transmission and tracing the source of infection of the organism is the effective way for control and prevention inside any medical institution in which MRSA became endemic. Accurate techniques of detection and characterization of MRSA are essential for appropriate antimicrobial therapy of infections. Many , molecular techniques have been established for typing MRSA that added a new reliable extent over the old simple techniques. Plasmid analysis was recently introduced in typing of MRSA. It was used in different epidemiological studies and helped investigators to classify their isolates and to identify the source of infection. Antibiogram is now considered one of the important available epidemiological tools for ”typing MRSA, and is widely used in different parts of the world by many investigators. The study of the external surfaces of the cell by lectins has been introduced as a new discriminatory technique in addition to the other techniques. IJ1yJresent studv aimed to: I Identify the role of MRSA in bacterial sepsis in the andria University Main Hospital. Evaluate the efficacy of some semi-selective media for the lation and rapid identification of MRSA. Identify the relatedness of the isolated strains of MRSA ugh different typing techniques. This study was done in Alexandria University Main Hospital ~ring the period from February 1996 to December 1997. One thousand subject of this study, they were divided into two First group: SOO inpatients with post-operative pyogenic wound Second group: SOO individuals, they were 62 health care workers and 438 inpatients. From the first group wound swabs were taken and were inoculated on each of blood agar, mannitol salt agar and macConkey agar plates. All plates were incubated at 37°C for 24 hrs. From the second group, nasal swabs were taken and streaked on each of OBA, CA, and OMA plates. All plates were incubated at 3SoC for 24 hrs. Identification of S,allreus colonies was based on coagulase production and V oges Proskauer test. MRSA were identified by the ability of growth on oxacillin ’eening plates (Mueller-Hinton agar with 6ug oxacillin / ml ) and by disc diffusion technique. Antibiotic susceptibility for MRSA isolates was determined by Baur and Kirby technique. MRSA characterized phenotypically by antibiotic were susceptibility test and by lectin binding assay, and genotypically by plasmid profile analysis. The results of this study showed that: I-Out of 500 infected cases, 210 (54.4) Gram negative bacilli, S.aureus and 75 (19.40/0) other Gram positive cocci were 2-MRSA represented 43.60/0 of the 101 S.aureus isolates. 3- A higher occurrence of MRSA infection was found among cases aged 41 years and more compared to other age groups. 4- The highest percentage of MRSA infection was among patients of bum and plastic surgery, diabetic and auto immune diseased patients.