الفهرس | Only 14 pages are availabe for public view |
Abstract Staph. aureus presents a potential pUblic health hazard as it is an extremely common cause of skin infections and many serious systemic infections as well as food poisoning. Staph. aureus is identified by the production of coagulase, thermonuclease, phosphatase, acid from anaerobic fermentation of glucose and mannitol and golden yellow pigmentation. Coagulase and thermo nuclease production are the most reliable practical criteria used in this identification. Three methods for detection of coaGulase have been used, the mout ::celiable one of them is tube method. However; the controversy on reading the test, the variability in plasma, the complications due to nonspecific reactions t and the incubation time have led to criticism of the test. Inspite of these shortcomings, the tube coagulase test is used routinely for the identification of Sta.ph.aureus. Also thermonuclease is claimed to be as specific as coagulase produc tion. This study was performed to find out the relation between thermonuclease production and grades of coagulase posH ivi ty. Other biochemical proper.ties used ir. identify ing Staph. aureus were also compared with the grades of coagulase test. .Antibiogram test was done for all the tested strains. This stuc~ was carried out on 100 Staph. aureus strains isolated and oolleoted from different clinical specimens; included wounds, ulcers, abscesses, sputum, urine, b |