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Abstract Human fascioliasis is becoming an important public health problem. In Egypt it has been reported from several provinces of the Delta, and outbreaks are extending in number and space. The clinical picture of human fascioliasis is highly variable. Specific diagnosis is based on recovery of the eggs in the patient’s stools, however negative microscopic examinations cannot exclude it. Moreover eggs do not appear in acute infection. Thus serology plays an important role in the diagnosis of fascioliasis. The key of success of any serological test is the development of satisfactory antigen and its use in a suitable test system. Various antigens, somatic or metabolic and a multitude of antigenic fractions prepared by advanced chemical techniques were assayed in animal hosts. Few studies were performed with human sera. The aim of the present work was to fractionate Fasciola adult worm antigen and to evaluate the different fractions in diagnosis of human fascioliasis by the ELISA technique. |