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Abstract 1- To diagnose pneumocystis carinii infection by light microscope , it is important to stain induced sputum and lung sample by one stain for the intracystic bodies like giemsa or gram stain , and another stain for cyst wall like to luidine blue O or gomori`s methenamine silver stain. 2- By using PAS stain , the parasite can only be recognized in smears but in lung sections, it is difficult to recognize it because it takes the same colour of the background. 3- Haematoxylin and Eosin only demonstrates the pathology caused by P. carinii infection in the lungs. 4- electron microscopical study of P. carinii is great value to detect the fine structures of the organism and to explain some morphological features of the parasite seen by light microscope, but it is difficult to do it as a routine work for the diagnosis of P. carinii. 5- the indirect immune fluorescent test (IFAT) is one of the best methods for diagnosis of pneumocystis carinii because it lung impression smears, bronchoalveolar lavage , paraffin and frozen lung sections. 6- elias method was found to be of value in serodiagnosis of P. carinii infection in human as the present work proved a statistically high percentage serpositivity in immunosuppressed and chest-diseased patient than in controls. |