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Abstract The aims of this study were to find out the prevalence of baoteriuria (symptomatio and asymptomatio) among a sample of primary sohoo1 ohi1dren in Alexandria, A.R.E.; to isolate and identify the different mioroorgan isms involved, and to determine the antibiograms for the isolated mioroorganisms. 2) This study (oros~-seotional) was oarried out on a total of 1250 ohildren from different areas of Alexandria (Eastern, Middle, W,estern and Rabaa). 3) Out of the 6250 ohi1dren examined, aged from 6 to 13 years, 631 were females and 619 were males. 4) Information was obtained from eaoh pupil regarding personal data, sooia1 aspeot, parents t eduoation and receipt of antimio1’obial therapy. 5) Midstream urine samples were oolleoted from the pupils in sterile oontainers. 6) The samples were examined maorosoopioally for colour and turbidity; and for the presenoe of prote in, glucose and pH value using a dipstrip (Med1-Test Combi 3). 11’, ” 7) The sediment of a 5’ ml. centrifuged urine was examined microscopically for the detection of pus cells and yeast like cells. 8) For urine culture, a sterile cooled platinum loop (calibrated to deliver 0.001 ml urine) was used to in ocu1ate each specimen onto (CLED) agar and (SDA) agar with cyclohexamide and chloramphenicol. 9) Bacterial count was done after 1-2 days of aerobic incubation at 37°C. 10) The significant Gram-positive cocci and Grampositive yeast like cells were identified by the usual methods, while the significant Gram-negative rods were identified by means of the Analytab Products, Inc. (API 20 E) system (see appendix). 11) The microorganisms isolated were tested for their susceptibility to antibiotics using the method of Stokes and Wa terworth (1972). The reference strain was Escherichia coli (NCTC, 10418). 12) Of the 1250 children examined, 360 (28.8) belonged to the high social class, 499 (39.92) to the middle class and 391 (31.28) belonged to the low social class. 13) Albuminuria was found in 38 (3.04) children. ~ne of these was found to. have significant bacteriu ria. 14) Out of the 770 centrifuged urine samples only 11 (1.43) yielded 5 or more pus cells per high power field. 15) There were twelve bacteriuric cases (0.96) (1.43 in girls and 0.48 in boys). The highest pre valence was at the age group 10 to 11 years (1.4). 16) Escherichia coli was the commonest isolate 7 (58.3), followed by Staph. species (Staph. aureus and Staph. albus).4 (33.4 ) and Klebsiella ox.vtoca 1(8.3). 17) Candida species was found in 13 (1.04) specimens. All the 13 specimens yielded a colony count less than 103 per ml. . 18) Nitrofurantoin and gentamicin showed the highest percentage of sensitivity to the bacterial isolat~ 12 (100). 19) Albuminuria was more prevalent 21( 5.83) in the children belonging to the high social class followed by the middle and low social classes,12(2.4) and 5 (1.28) respectively. |