الفهرس 
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Abstract
Forty eight water samples were collected from four sites in Mit
Mazah sewage treatment plant at Dakahlia governorate. Samples were
collected during (May 1991 to April 1992).
Effiuent of two step treatment system
1- Influent of an oxidation or Maturation pond.
2- Maturation pond effiuent discharge befor mixing with the receiving
Agiculture drain water.
3- 100 meter upstream, before the point of discharge.
4- 100 meter down stream, after discharge point
The results showed that all sampling locations were strongly
dominated by four Taxonomic orders :-
order : Hemiptera were presented by 39.8% along the all season.
order : Odonata were presented by 31.7% along the all season.
order : Diptera were presented by 18% along the all season.
order: Coleoperta .• were Presented by 1I .6% along the all season.
•
For virological study two methods of virus concentration were
carried out to evaluate the effeicency of different membrane filters for
enteroviruses recovery from wastewater. The results showed that no great
difference in Nitrocellulose membrane effeicency with and without
conditioning where the percentage of recovery were 40% and 30%
respectively.
In case of glass micro fiber filter the percentage of recovery were
40% and 20% respectively.
The percentage of virus recovery were the same after conditioning;
So we started the concentration process with nitrocellulose membme.
To increase the possibility of virus isolation, three types of cell
cultures were used, African green Monkey Kidney (Vero) cells, Buffiuo
green Monkey (BGM) cells, and Mosquito cell line C636.
The results showed that BGM cell lines were more sensitive and
speed than vero cell lines enteroviruses isolation, while mosquito cell lines
C636 were not sensitive.
DOT-ELISA was used to detect enteroviruses ill cell culture
harvests at the third passage in BGM cell. The results showed that
Poliovirus type III was dominont in somples (37.2%) followed by
Coxsackievirus type B4 (21.5%), Poliovirus type I (16.6%) Poliovirus type
II (13.7%), Coxsackievirus A6 was detcted in 10.7% of the investigated
samples. Rotavirus was not detected in all samples. So the wastewater
treatment plant faild to remove most enteric viruss investigated.
The results showed the need for more efficient disinfection step for
the treated efl’uents. Chlorine, Ozone, and Ultraviolt radiation were used
for virus inactivation. Complete inactivation of Poliovirus type II (Salk
strain) obtained at chlorine dose of 20 mgIL (residua chlorine was 1.30
mgIL) detention time was 30 minutes, While the dose for inactivation of
Coxsackievirus B4 was 30 mgIL .
In case of Ozone treatment, the doses of ozone used for Poliovius II
inactivation were 319 mgIL/h for 17 min for seeded effluent sample, were
• as for Coxsackievirus B4, the dose must be over 21 min.
In case of the effect of ultraviolet exposure time for disinfection of
poliovirus type II and Coxsackievirus B4 were the same 20 secound for
seeded effluent samples.
Regression analysis of Polioviruses type II (salk strain) and
Coxsackievirus B4 revealed that : with increasing doses of different
disinfectants, increase the inactivation effieciency of different viruses.
Revealed high regression coeffiecient, this means that Ozone is the most
suitable disinfectant for Coxsackievirus B4 and Poliovirus type II were the
regression coefficient (~) reached to 0.72 for Coxsackievirus B4 and 0.92
for Poliovirus type II.