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Abstract - The ability of microbial communities of nine soil, sludge and water samples to grow on 7 different chloroaromatic compounds were determined. - Microbial community of soil ”3” showed the highest growth on ,1mM of l,4-dichlorobenzene. - Indigenous microbial population of soil ”3” revealed the highest growth on 1mM was recorded on 4-chloroaniline. - Growth on 1~1 of 4-chlorophenol indicated that soils 1,2,4 and 8 .gave the best results. - The best grown communities on 1mM of chlorobenzene were that of soils and sludge 1,3,4 and 8. - Microbial populations of soil ”3” recorded the best growth on 1mM of 2-chloroaniline. - Indigenous populations of soils 8,1 and 4 revealed the best growth on 1mM of 1,2-dichlorobenzene. - Microbial population of sludge grow well on IO~M of 2,6- dichlorophenol indolphenol sodium salt. - Indigenous populations of soil ”3” recorded the best growth on 3mM of 4-chloroaniline. - Microbial populations of soil ”1” gave the best growth on 3mM of 4-chlorophenol. - Microbial populations of soil ”8” gave the best growth on 3mM of chlorobenzene. 141 _ All the four microbial populations of 2,3,4,8 grew slightly on 3mM of 2-chloroaniline. _ Microbial populations showed to some extent growth on 3mM of 1,2-dichlorobenzene. _ Microbial communities of soils and sludge gave a good growth on 1OO~Mof 2,6-dichlorophenol indolphenol sodium salt greater than those of water samples which also gave good results. _ The best growing population was that of soil ”3” on SmM of 1,4- dichlorobenzene. _ . Also soil ”3” revealed the best growth on 5mM of 4-chloroaniline. _ Growth of different populations on 3mM and smM were fast at the first 7 days and it decreased as the concentrations increased. _ Count of populations grown on ImM of 1,4-dichlorobenzene and 4-chloroaniline were higher than that on 3mM which were subsequently higher than that on 5mM. _ In general count of lmM was higher than that of 3mM in all microbial populations degrading 4-chlorophenol, chlorobenzene, 2- chloroaniline and 1,2-dichlorobenzene. 120 colonies were characterized isolated from different soil and sludge samples on different chloroaromatic compounds. _ Only 31 colonies were able to grow on SOO~Mand 1OOO~ of 2,6- dichlorophenol indolphenol sodium salt. _ The most promising colonies capable of growing on 1000J.1Mof 2,6-dichlorophenol indolphenol sodium salt were exposed to different doses of garruua irradiation. 142 _ 3 KGy reduced the count 9.4, 9.7, 10.2, 8.2 log cycles to the isolates MAM-l06, MAM-J07, MAM-I09, MAM-94 respectively and reduced the viability completely to MAM-I08, MAM-) 04 isolates. _ 4 KGy reduced the viability of MAM-95 completely, 5 KGy reduced the viability ofMAM-89 by 6.7 log cycles, 8 KGy reduced the viability of MAM-IOI completely, while 10 KGy reduced the count ofMAM-96 by 2.5 log cycles. _ Control of all the ten isolates were able to grow on 2mM of 2,6- dichlorophenol indolphenol sodium salt, while the growth of the isolates exposed to the gamma irradiation was stronger than control. _ Non of the ten isolates (control) able to grow on 3mM of 2,6- dichlorophenol indolphenol sodium salt, while some colonies of these isolates which exposed to gamma irradiation able to grow on this concentration. _ The most grown isolate on 8mM of 1,4_dichlorobenzene, 4- chloroaniline, 2_chloroaniline, l,2_dichlorobenzene and 4- chlorophenol was MAM-96 which represent Bacilli, while its control can’t grow on 8mM of 4_chlorophenol. _ Isolate .MAM-I 04 can’t grow on 8mM of 4-chlorophenol while its mutant exposed to O.5KGy can grow, Isolate MAM-I08 can’t grow on 8mM of both 2-chloroaniline and 1,2-dichlorobenzene but its mutant exposed to 0.5, \.0, \.5KGy can grow, mean while, MAM- 106 can’t grow on 8mM of both 1,4_dichlorobenzene and chlorobenzene but its mutants exposed to 0.5 and 1.0KGy able to grow. 143 MAM-96 was chosen to detenninc the analytical analysis using HPLC. - MAM-96 remove 77% of ImM of 2,6-dichlorophenol indolphenol sodium salt after 15 days. - MAM-96 remove 98.5%, 95.3%, 98.3%, 100%, 100% and 15% from 3mM of lA-dichlorobenzene, 4-chloroaniline, 2- chloroanilir.e, 1,2-dichlorobenzene and ·chlorobenzene respectively after 15 days. - The ten chosen strains grown on 3mM of different chloroaromatic compounds were scanned by electron microscope. |