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العنوان
biodegradation of haloarmatic compounds/
الناشر
ola abd el aal abd el atty,
المؤلف
khalil،ola abd el aal abd el atty
هيئة الاعداد
باحث / oal abd el-aal abd el-atty
مشرف / nagy h.aziz
مناقش / mahmoud swalim
مناقش / nagy h.aziz
الموضوع
botany
تاريخ النشر
2005 .
عدد الصفحات
143p.:
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
1/1/2005
مكان الإجازة
جامعة بنها - كلية العلوم - علم النبات
الفهرس
Only 14 pages are availabe for public view

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Abstract

- The ability of microbial communities of nine soil, sludge and water
samples to grow on 7 different chloroaromatic compounds were
determined.
- Microbial community of soil ”3” showed the highest growth on
,1mM of l,4-dichlorobenzene.
- Indigenous microbial population of soil ”3” revealed the highest
growth on 1mM was recorded on 4-chloroaniline.
- Growth on 1~1 of 4-chlorophenol indicated that soils 1,2,4 and 8
.gave the best results.
- The best grown communities on 1mM of chlorobenzene were that
of soils and sludge 1,3,4 and 8.
- Microbial populations of soil ”3” recorded the best growth on
1mM of 2-chloroaniline.
- Indigenous populations of soils 8,1 and 4 revealed the best growth
on 1mM of 1,2-dichlorobenzene.
- Microbial population of sludge grow well on IO~M of 2,6-
dichlorophenol indolphenol sodium salt.
- Indigenous populations of soil ”3” recorded the best growth on
3mM of 4-chloroaniline.
- Microbial populations of soil ”1” gave the best growth on 3mM of
4-chlorophenol.
- Microbial populations of soil ”8” gave the best growth on 3mM of
chlorobenzene.
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_ All the four microbial populations of 2,3,4,8 grew slightly on 3mM
of 2-chloroaniline.
_ Microbial populations showed to some extent growth on 3mM of
1,2-dichlorobenzene.
_ Microbial communities of soils and sludge gave a good growth on
1OO~Mof 2,6-dichlorophenol indolphenol sodium salt greater than
those of water samples which also gave good results.
_ The best growing population was that of soil ”3” on SmM of 1,4-
dichlorobenzene.
_ . Also soil ”3” revealed the best growth on 5mM of 4-chloroaniline.
_ Growth of different populations on 3mM and smM were fast at the
first 7 days and it decreased as the concentrations increased.
_ Count of populations grown on ImM of 1,4-dichlorobenzene and
4-chloroaniline were higher than that on 3mM which were
subsequently higher than that on 5mM.
_ In general count of lmM was higher than that of 3mM in all
microbial populations degrading 4-chlorophenol, chlorobenzene, 2-
chloroaniline and 1,2-dichlorobenzene.
120 colonies were characterized isolated from different soil and
sludge samples on different chloroaromatic compounds.
_ Only 31 colonies were able to grow on SOO~Mand 1OOO~ of 2,6-
dichlorophenol indolphenol sodium salt.
_ The most promising colonies capable of growing on 1000J.1Mof
2,6-dichlorophenol indolphenol sodium salt were exposed to
different doses of garruua irradiation.
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_ 3 KGy reduced the count 9.4, 9.7, 10.2, 8.2 log cycles to the
isolates MAM-l06, MAM-J07, MAM-I09, MAM-94 respectively
and reduced the viability completely to MAM-I08, MAM-) 04
isolates.
_ 4 KGy reduced the viability of MAM-95 completely, 5 KGy
reduced the viability ofMAM-89 by 6.7 log cycles, 8 KGy reduced
the viability of MAM-IOI completely, while 10 KGy reduced the
count ofMAM-96 by 2.5 log cycles.
_ Control of all the ten isolates were able to grow on 2mM of 2,6-
dichlorophenol indolphenol sodium salt, while the growth of the
isolates exposed to the gamma irradiation was stronger than
control.
_ Non of the ten isolates (control) able to grow on 3mM of 2,6-
dichlorophenol indolphenol sodium salt, while some colonies of
these isolates which exposed to gamma irradiation able to grow on
this concentration.
_ The most grown isolate on 8mM of 1,4_dichlorobenzene, 4-
chloroaniline, 2_chloroaniline, l,2_dichlorobenzene and 4-
chlorophenol was MAM-96 which represent Bacilli, while its
control can’t grow on 8mM of 4_chlorophenol.
_ Isolate .MAM-I 04 can’t grow on 8mM of 4-chlorophenol while its
mutant exposed to O.5KGy can grow, Isolate MAM-I08 can’t grow
on 8mM of both 2-chloroaniline and 1,2-dichlorobenzene but its
mutant exposed to 0.5, \.0, \.5KGy can grow, mean while, MAM-
106 can’t grow on 8mM of both 1,4_dichlorobenzene and
chlorobenzene but its mutants exposed to 0.5 and 1.0KGy able to
grow.
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MAM-96 was chosen to detenninc the analytical analysis using
HPLC.
- MAM-96 remove 77% of ImM of 2,6-dichlorophenol indolphenol
sodium salt after 15 days.
- MAM-96 remove 98.5%, 95.3%, 98.3%, 100%, 100% and 15%
from 3mM of lA-dichlorobenzene, 4-chloroaniline, 2-
chloroanilir.e, 1,2-dichlorobenzene and ·chlorobenzene respectively
after 15 days.
- The ten chosen strains grown on 3mM of different chloroaromatic
compounds were scanned by electron microscope.