الفهرس 
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Abstract
Metarhizium anisopliae, Metarhizium flavovridae, Beauveria
bassiana, Beauveria brongniartii and Paecilomyces farinosus
were used as a biological control agents for the Egyptain cotton
leaf-worm Spodoptera littoralis (Boised.), which represents the
most severe destructive cotton pest in Egypt and many other
countries and leads to a decrease in the national income of our
country and pink bollwonn was reported as a serious pest of
cotton in 1940, it has since become the most injurious insect
pest cotton in many cotton-growing regions of the world.
The main points which were discussed in this study could be
summarized in the following topies:
I-Mass production of the five fungal isolates under study in
different growth media.
2-Efficiency of the fungal isolates as a biological control agents
against the Egyptain cotton leaf worm S.littoratis and
P.gossypiella under laboratory conditions.
3-Protein analysis for fungal isolates by sodium dodocyle
sulphate polyacrylamide gel electrophoresis (SDS-PAGE)
4-Determination of aflatoxins by Thin layer chromatographic
(TLC) and High performance liquid chromatography (HPLC)
The fungi under study were grown on three different media
.where M.anisopliae, J.f.jlavovridae, B.bassiana and
B.brongniartii exhibits good growth on Yeast Extract Sucrose
whereas Paecilomyces farinosus exhibit good growth on
Czapek’s-Dox Agar medium. The medium constituents were
prepared and adjusted at pH 5.5-6.5. After sterilization, Petridishes
were inoculated with the fungal strains and incubated for
two weeks at 25° C&50-60% RH. At the end of the incubation
period, the supernatant was separated from the mats. Three
concentrations were used viz 40, 60 and 80%, these
concentrations were made by using ammonium sulphate.
The 1st instar larvae of P.gossypiella were mixed in artificial
diet with fungal toxin 40, 60 and 80%. from each concentration
take I ml and homogenately mixed with 15 gm of artificial diet.
The infected larvae were placed in sterile cups containing
artifical diet, ten hatched larvae of P.gossypiella were placed on
the surface of the diet, all stages were reared and treated under
constant conditions of 27±1DC and 80±5%RH. Mortality counts
were recorded after 48h of treatments, mortality percentage of
Manisopliae after 9 days of treatment was 85.970%. Generally
Manisopliae was found to be the most effective isolate against
1st instar larvae of P.gossypiella followed by B. bassi ana,
P.farinosus, Mflavovridae then B.brongniartii.
The previous same techniques were used except for all stages
were reared and treated under constant conditions of 25± 10C
&50-60%RH, mortality percentage of B. brongniartii after 12
days of treatment was 83.36%. Generally B. brongniartii was
found to be the most effective isolate against I” instar larvae of
Slittoralis followed by Pfarinosus, M.jlavovridae,
M anisopliae then B. bassiana.
Mortality percentage of B. bassiana after 12 days of treatment
was 78.53%. Generally B. bassiana was found to be the most
effective isolate against 2 nd instar larvae of S.UttoraUs followed
by Pfarinosus, B.brongniartii, ManisopUae, M.jlavovridae.
The proteins of five isolates mentioned above was extracted
from their supernatant and fractioned by SDS-PAGE. SDSPAGE
revealed a maximum number of 9 bands, which not
necessarily present in all samples. For B.brongniartii there are
five bands at conc.80%, four bands at conc.40, 60%. For M
anisopliae there are three bands at conc.80%, two bands at
conc.60% and one band at conc.40%. In addition to
Mflavovridae, P.farinosus and B.bassiana appeared (5, 6, 9)
bands at cone. 40, 60, 80% for Mj/avovridae, (3, 4, 5) bands at
cone. 40, 60 and 80% for P.farinosus, and (1, 1, 2) bands at
concAO, 60 and 80% for B.bassiana. Concentration 80% for all
fungi was characteristic by large number of bands then 60, 40%.
The five tested entomopathogenic fungi can not produced any
types of aflatoxins B1, B::;,G1 and G2 by (TLe) and (HPLC).