الفهرس 
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Abstract
SUMMARY
A number of 96 immature and adult sheep of both
sexes were used in our experiment. These animals were
classified into 6 equal groups ot”both sex and age,
with 16 animals in eac’hgroup. One group was used as
control and injected with 1.0 ml olive oil only. The
other 5 groups were injected.with B-sitosterol in the
following doses (0.05, 0.25, 0.5, 0.75 and 1.0 mg/kg
body weight) for 4 weeks. Injection continues daily
for 4 weeks. Blood s~ples were taken from all animals
at weekly intervals during the whole experiment. In the
present study an attempt has been made to determine the
effect of B-sitosterol on both calcium and phosphorus
as well as the levels of parathormone and calcitonin.
In addition the effect of B-si~osterol on the levels
of aldosterone, ACTH, Sodium and Potassium were also
studied.
i- Effect on serum calcium:
In male lambs, a significant rise appeared after
three weeks following the doses 0.25 mg and 1 mg
B-sitosterol.~ The same effect produced after the fourth
week with all the doses of B-sitosterol eXQept 0.05 mg
dose.
In femal.elambs, after one week’, ~iL8ignificant
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increase in serum calcium appeared following the 1 mg
dose. The increase was also observed after the third
week: with doses 0.5 mgt 0.75 mg and 1 mg. After the
fourth week, all doses of B-sitosterol elevated calcium
significantly.
In rams, the significant increase was produced
only after four weeks with the doses 0.25 mg, 0.5 ’rug
0.75 mg and 1 mg.
In ewes, the calcium increased significantly after
four weeks only.
2 - Effect on serum phosphorus :
The phosphorus level in the ;.,serumwaS not significantly
changed following the administration of
B-sitosterol for one, two, three and four weeks application
in male and female lamb~ rams and ewes.
3 - Effect on serum sodium :
Sodium level in the serum following the different
doses of B-sitosteril was changeable according to the
sex and age of the tested animal and also the dose concentration
as well 8S the time of application of
B-sitosterol.
.
4 - Effeot on serum Potassium :
The different doses ofB-sitosterol produced a
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slight non significant decrease in the level of
serum Potassium after one, two three and four weeks
in immature and mature sheep.
5 - -Effect on serum parathormone :
The effects ofdifferent doses of B-sitosterol
on the different serum parameters of the injected
lambs, sheeps and ewe, were obviously changeable
in accordance to the dose, concentration, age and
sex as well as duration of treatment.
In male lambs, there was no Significant rise
in hormone level following the administration of
B-sitosterol for one, two and three weeks while
after four weeks a significant increase appeared
as an effect of dOses 0.75 mg and 1 mg B-sitosterol.
In female lambs a significant increase observed
especially atter the third week for the dose of 1mg
B-sitosterol. The same increase ,appeared also after
fourth week ot application at doses of 0.75 mg and
1mg B-sitosterol.
In rams the increas~ in parathormone was significant
atter the doses of 0.75 mg and.-.lmg B-si tosterol at .. .
the third waek aftd after 0.5 mg. 0.75 mg and 1 mg doses
at the fourth week.
In ewes, a significant· rise in paratho~one level
appeared only after the fourth week. at doses 0.75 mg
and 1 mg.
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6 - Effect on serum ca1citbnin :
The effect of B-sitostero1 on calcitonin revealed
that the effects following the administration of
different doses of B-sitosterol for different periods
of application 1, 2, 3 and 4 weE;~s were as follows :-
..
- In male lambs,~the decrease in calcitonin level
was not significant after the injection of B-sitosterol
in different doses during the weeks of treatment.
In female lambs the serum level of calcitonin indicated
a slight decrease after administration of
B-sitosterol for four weeks.
- Moreover in rams and ewes a non significant decrease
in calcitonin level was observed following the treatment
with various tested dose, of B-sitosterol afte~
1 ,2, J and 4 weeks.
7 - Effect on serum aldosterone :
Administration of different doses of B-sitosterol
produce same variations in the· responae of both sexes
of immature and mature sh~~p.
In male ~ambs, the increase in aldosterone level
was significant after two, three and four weeks following
the last largest dose only.
In female lambs, the aldosterone level was raised
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significantly after four weeks for the doses 0.5 mg,
0.75 mg and I mg B-sitosterol.
In rams, the injection of B-sitosterol,for one
and three weeks resulted in a significant rise following
the injection of 1 mg B-sitosterol while 0.75 mg
and the lmg doses produced significant increase after
fourth week •.
In ewes, the highest concentration of 1 mg
B-sitosterol resulted in a significant increase after
two and three weeks. Furthermore all the injected
doses of B-si tosterol except 0.05 mg and ’0.85 mg
doses eXhibited a significant increase after the fourth
week.
8 - Effect on serum ACTH:
The effect of B-sitosterol on the level of ACTH
in lambs and sheep was variable.
In male lambs, a significant rise in hormone
level was seen following treatment with doses of 0.5 mg
and 1 mg B-sitosterol at the second week and atter 0.75 mg
and 1 mg’doses at the third week. Furthermore _ significant
increase appeared after the highest dOS8 of 1 mg
B-sitosterol after the fourth week.
In female lambs, after the first and second week
there was a signi·,ficant rise with the dose” ot 1 mg
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.’.
...•..•
B-sitosterol. Significant increase waS observed after
the third week with doses of 0.75 mg and lmg B-sitosterol.
After tour weeks of application the increase in ACTH
level was highly significant especially with 1mg dose.
In rams the doses of 0.75 mg and 1 mg B-sitosterol
induced a significant increase in plasma ACTH after two
and three weeks. While after four weeks a significant
rise in hormone level appeared after 0.25, 0.5 and 0.75
mg doses. The increase was highly significant by 1 mg
dose.
In ewes, the last doses of 0.75 mg and 1 mg of
B-sitosterol produced a significant rise after the
first week. The same effect was produced’ after two
weeks by the doses of 0.5 mg and 1 mg. After three
weeks, the doses 0.5 mg, 0.75 mg and 1 mg produced significant
increase in ACTH which was highly significant
after the fourth week.