الفهرس 
Materials and methodsOnly 14 pages are availabe for public view
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Abstract
This work was aimed of detection of C. trachomatis by direct methods
in non pregnant women of the child bearing period. These laboratory
techniques were:
1- Giemsa stain technique.
2- Direct immuno fluorescence technique.
3- Tissue culture technique using BGM monolayer cells.
Group I : 200 women suffered from some lower genital tract symptoms
and/or signs were taken as cases.
while Group \I 100 women not complaining from any lower genital tract
symptoms or signs were taken as control.
Study groups (cases and controls) were divided into subgroups
according to symptoms, signs, age, residence, number of abortion(s), using
of contraceptive method, week of menstrual cycle, and fertility condition.
It is concluded that Tissue culture technique for detection of C.
trachomatis is better than Giemsa stain and DFA techniques.
C. trachomatis infection were present in both cases and controls.
Prevalence of positive results of C. trachomatis were significantly lower in
control group.
Younger women were more positive for C. trachomatis infection.
Women from rural areas were more significantly positive for C.
trachomatis infection.
Women using contraceptive pills were more significantly positive than
those using other contraception methods.
Detection of C. trachomatis were more significantly positive during
second and third week of menstrual cycle.
Infertile women were more significantly positive for C. trachomatis
infection.
Women had abnormal cervix were more significantly positive for C.
trachomatis infection.
Recommendations
It is better to use more than one methode for diagnosis of chlamydial
infections to get the actual results about the disease, evaluate the
magnitude of the problem, and then establish the ideal lines for eradiction of
this agent from the community.
Chlamydial infection is notoriously a disease of poverty, lack of water,
and poor hygiene. Initiating efforts to control this disease requires an
awareness of the problem by both public health authorities and the
population concerned. The success of mass campaigns depends greatly on
education, finance and politics.
Furthur studies should be carried out to assess the Chlamydial role in
genital infections in our community.
Although tissue culture is the method of choice for identificaiton of
endocervical chlamydial infection, immunofluorescence test can be used as
an alternative method to tissue culture for detection of C. trachomatis
infection in high prevalence women. The DFA test appears to be rapid, it
could be done within 30 minutes. Careful training in reading DFA smears,
will be critical in the resulting sensitivity and specificity of the test. Pearing
how to recognize artifacts and to differentiate them from elementary bodies,
reticulate bodies is of importance.