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Abstract -86- SUMMARY This work evaluated streptococcal grouping by means of slide agglutination test using specifically sensitized latex part ides after enzymatic extraction of group specific carbohydrate antigen is more simple rapid and reliable method for groupings. Among 44 selected streptococcal isolates from upper respiratory tract infections, wound infections and gentio urinary infections, these selected strains picked up, identified primiarly by colony morphology, type of haemolysis and by films these were grouped by latex the result of this groupind were 18 isolates of group A, 6 isolates group B, 5 isolates group C and l( isolates group D. * The groups A,B and C were beta haemolytic. * Haemolysis was variable in group D isolates. Each group tested was confirmed by its own presumtive tests avaliable. * For group A isolates: bacitracin sensitivity were done 15 isolates were sensitive and 3 isolates were resistant and the correlation of bacitracin with L.A was 83.3%. -87- * For group B isolates: all of them hydrolyze hippurate and the correlation was 100%. * The group C not tested. * For group D all strains gives positive bile salt tolerance and 6.5 Nacl tolerance which indicates that all isolates were enterococcal and the correlation was 100%. The overall correlation of strains tested with their presumptive testes used from 83.3 to 100%. from the above we conculds that latex agglutination test (Streptex) is more simple, rapid and reliable method for grouping streptococci. |