الفهرس 
introductionOnly 14 pages are availabe for public view
 |  | from | 194 |  |  |
| | | from | 194 | | |
Abstract
The World Health Organization (WHO) estimated that human tuberculosis incidence and death for 1990 to 1999 will be 88 million and 30 million respectively, with most cases in developing countries. Zoonotic tuberculosis (caused by Mycobacterium bovis) is present in animals in most developing countries where surveillance and control activities are often inadequate or unavailable; therefore, many epidemiologic and public health aspects of the infection remain largely unknown.
The reported high incidence of bovine tuberculosis in Africa and especially Egypt, has stimulated us to search for tuberculous cases caused by Mycobacterium bovis among tuberculous patients in Cairo and El-Giza Chest Hospitals as follow:
One handred tuberculous patients (extra pulmonary TB only) with positive history of direct contact with animals such as butchers, animal feeders, and rural workers were selected from Cairo and El-Giza Chest Hospitals. They were subjected to history taking, X-ray films, tuberculine test, tissue biopsies and complete blood counts.
Specimens were subjected to the following examinations:
•Culture on Lowenstein - Jensen medium
•Colonies grown on cultures were subjected for oxyR allele specific PCR technique for differentiation between Mycobacterium tuberculosis and Mycobacterium bovis.
*Results:
•Male patients represented 61% (61 patients), while female patients represented 39%(39 patients).
•The highest incidence of extra-pulmonary tuberculosis was observed among patients between the age of 20-40 (40 cases- 40%), above the age 40 years (39 cases-39%) and the lowest incidence was among patients below the age of 20 (21 cases- 21%).
•Cultures on Lowenstein-Jensen medium were positive for 46%(46cases) of samples and the rest of samples 54%(54cases) were negative because inappropriate storage, improper transportation, bad medias and lastly due to bad growth of the organisms on LJ media.
•oxyR allele specific PCR tests were, 100% oxyR/H positive, 0 % oxyR/B negative which done on only 46 cases which are positive cultures and no samples gave any reaction to oxyR/B.
All cultures were identified as Mycobacterium tuberculosis and non of them was Mycobacterium bovis.