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Abstract
Many Trichoderma aggregate species are of economic interest because of their ability to produce hydrolytic enzymes. Trichoderma offer the complete set of cellulases in the right amounts under the right conditions to achieve the hydrolysis process. Consequently, the main objective of the present study is improving Trichoderma isolates for successful application in biological control domain and bio-industry. Thus, it is useful to mutagenize different Trichoderma aggregate species to maximize its capacity as biocontrol agent and their cellulase activities. To accomplish our goal, first thirteen cultures of T-richoderma were isolated from lignocellulosic agriculture wastes rich soil. These isolates were morphologically differentiated as T. koningii, T. hamatum and T. harzianum, To confirm this finding, genetic similarity based on PCR-RAPD data was applied to NTSYS-pc program to obtain genetic distances among Trichoderma isolates. The obtained dendrogram indicated that the clustering of both T. koningii and T. hamatum aggregate species were consistent with data obtained from the morphological identification of Trichoderma species with two exceptions. These results led us to conclude that random amplified polymorphic DNA patterns can provide reliable tools for Trichoderma grouping. The thirteen isolates were evaluated for their extracellular enzyme activity. Two analyses were used, the first one is extracellular-cellulases activity and the second is the antagonistic ability toward the soil-borne pathogen that contains cellulose as the main cell wall component. The extracellular hydrolytic cellulases enzyme, FPase, p-l,4-endoglucanase