الفهرس 
Acute Iy mpho blastic Zen kemiaOnly 14 pages are availabe for public view
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Abstract
Acute lymphoblastic leukemia is the most common form of childhood leukemia. Genetic alterations in childhood leukemias are powerful prognostic indicators. The TFIAMLI fusion gene created by the t (12;21) is the most common genetic alteration in childhood acute lymphoblastic leukemia and is associated with favorable prognosis, less aggressive course, long survival rate, longer event free survival, and late relapse if happened. In the light of this, the present work aimed to detect the frequency and prognostic significance of TEI-AML 1 fusion gene among Egyptian childhood B-lineage acute lymphoblastic leukemia, and to evaluate FISH and PCR as methods for detection of this translocation. Peripheral blood were obtained from 50 children suffering from ALL (all of them were newly diagnosed). A- fter culture technique and RNA extraction, only 29 patients were available and all were subjected to the following: Full history and thorough clinical .examination. CBC Bone marrow aspiration. Cytochemistry of the peripheral blood and bone .marrow films. Immunophenotyping FISH using LSI, TEL-AML1 dual color DNA probe for 29 cases only. RT-PCR for 29 cases only. The results of the studied patients using FISH and PCR are summarized as follows: Three patients were positive for TeVAMLl (10.34%) using both FISH and PCR while 26 patients were negative for TELIAML 1 (89.66%). The age of the positive patient, for TELIAML1 range between 3 - 8 years with a mean of 5.66*2.44 years while the age of the negative patients for TELIAML1 range between 2.5 - 15 years with a mean of 7.8 1 -+ 4.37. As regard sex, 2 male patients were positive for TEL/AMLl (66.67%), while only one female was positive for TELIAMLl (33.33%), while 18 male patients were negative for TEL/AMLl (69.20%) and 8 females were - negative for TELIAML 1 (30.8%).