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Abstract Thirty eight accessions of three species of the genus menthe ( Mentha longifolia, menthe spicata and menthe piperita) and seven accessions of two species of the genus Ocimum (ocimum basilicum and Ocimum Kilimandscharicum ) in Egypt were analyzed to assess the genetic diversity levels within and among populations and species of both genera, three types of traits were used in this study; morphological traits, seed proteins elecrophoretic profiles under reducing conditions and variations in isozyme electrophoretic profiles on polyacrylamide gels. Morphological variation was not completely sufficient to distinguish different species or illustrate pattern of infraspecific variation; probably due to the use of few number of morphological traits. The data obtained from polyacrylamide gel elecrophoresis of seed proteins clearly separated menthe spicata from menthe longifolia. The analysis of isozyme polymorphism in both menthe and Ocimum clearly separated different species, Mentha Piperta was clearly delimited from Mentha Spicata and Mentha longigolia, accessions of the latter two species were also delimimted as two separate groups. Both, seed proteins and Isozymes Data Also Revealed impact of geographic distribution on the genetic diversity particularly in Mentha Longifolia and Ocimum basilicum. In Mentha longifolia accession representing the delta region showed closer similarity to each other and accessions collected from sites outside the Delta were also Similar to each other. One accession representing Mentha longifolia subspecies schemperi was not delimited as a separate identity. |