الفهرس 
REVIEW OF LITERAUTEROnly 14 pages are availabe for public view
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Abstract
Szim mai’ and 6rrnelusIons
This work had been presented to evaluate the DNA content (DNA ploidy and index, P53 gene and serum CEA abnormalities, to study the relation between these abnormalities and other clinicopathologic prognostic parameters and to demonstrate the prognostic value of these parameters in 40 patients with colorectal carcinoma presented to Menoufia Ui iversity Hospital, Darnanhour Medical National Institute And Darnanhour Oncology Center during the course of study.
Three sections 30,20, 5 Micron thick sections were obtained from each specimen prepared for detection of DNA content abnormalities by flow eytornetry, detection specific sequence of DNA by immunohistochemical analysis to detect P53 gene mutation and staining with haematoxylin and eosin to confirm the 4iagnosis. Blood sample was taken for detection of the serum CEA level for each patient.
For each patient, data about age, sex site of the lesion, histopathological type grade of differentiation, pathological stage and lymph node status were obtained. Patients were followed up till the end of the course of the study ( 4-36 months).
In the present work, colorectal cancer has been aneuploid in 27 patients (67.5% ) while 13 (32.5%) were diploid. DNA aneuploidy and high DNA index (DI > 1.4), have been significantly higher in rectal and left colon cancer, poorly differentiated colorectal carcinomas, No significant association has been found between either age or sex of the patients, extramural venous invasion and histological types of tumors and DNA index or ploidy pattern.
Tumor stages (TNM or Ducks’ stage), lymph node involvement, CEA level and P53 mutation showed no statistically significant difference as regards DNA ploidy, however, Dl has a strong relation to these parameters.
We observed P53 mutation frequency of 47.5% (19 out of 40) with significant relation between p53 and all pararnetes except age, sex and extramural venous invasion.