الفهرس 
Separation of complex lipidsOnly 14 pages are availabe for public view
 |  | from | 150 |  |  |
| | | from | 150 | | |
Abstract
The effect of l i g h t on various aspects of growth has been studied in Verticillium agaricinurn. The fungus produces spores urrlder all wavelengths tested (300- 79 nm) as well as i n the dark. Hear-W markedly increases sporulation and c e l l hall thickness, while it retards gr0hit.h and spore germination. Total lipids increase all irradiations used while complex lipids are significantly increased under near-W, black, blue and red irradiations. The presence of f a i r l y large arr.oimts of lipids in the dark suggests tbat l i p i d synthesis is not under ~hotocontrol. However l i g h t is e s s e n t i a l f o r carotenoid synthesis i n -V. =richan. Carotenoids i d e n t i f i e d i n -V. agaricinurn under near-UV a r e ; B-, 5-, and 8-carotenes, neurosporene, torulene, neurosporaxanthin and its methyl ester. Evidence su?ports the suggestion that %carotene and not torulene, is the irnrnefiiate precursor of neurosporaxanthin. A crude action action spectrun for photoinduction of carotenoid synthes i s a t high i r r a d i a t i o n has a F~~KULFpp.c l-k a t tine near-W region with smller pezks i n the blue, yellow red regions. Short exposures t o red irradiations increases ca.rotenrj-6enesis while t h i s effect is reversed by *-red irradiation. This together kith other evidence, including a far-red minus red difference spectrum of crude extract of the fungus (absorption maximum 670 nm and absorption minimum 750 nm) indicate that phytochroiae may be involved here. The irradiance received at these two mvelengths is very important to this fungus. A t low imdimce a 2-ed, fw-red effect is obvious bit at high irradiance t h e red effect is pl-actically non existent presumably due t o singlet oxygen. The action spectrum for carotenogenesis in 1. agaricinurn has a maxima at 395 and 660 nm with two lesser peaks at 433 and 730 nm. A crude pigment extracted from x. agarichim ( 260, 370/375 and 440 nm) may be the pigment which photoregulates carotenoid synthesis in the near-UV while phytochrome is the one in the red, farred regionof the spectrum.