الفهرس 
Biochemical aspects of schistosomiasis mansoniOnly 14 pages are availabe for public view
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Abstract
Chronic bilharzial liver disease is considered one of the most important health problem in Egypt. Though many chemotherapeutic agents have been used to control this disease,no effective measure was found to improve the status of the liver after antibilharzial treatment. Thiola,on the other hand, is a new synthetic sul fhydryl compound that has been reported to be markedly effective in various disorders involving liver diseases especially alcoholism. Hence, the present study was performed to evaluate the effect of thiola on the status of mice infected with S. mansoni and pre-treated with - the antibilharzial drug praziquantel. Investigation has been performed on two di fferent progressive stages of the disease ; an early stage of 7 week infection and a late stage of 14 week infection and the effect was followed up biochemically by determination of some liver enzyme activities namely ; alkaline phosphatase, glucose-6-phosphatase, 5’-nucleotidase, acetylchol oxalacetic any, andto inesterase, glutamic pyruvic transaminase and glutamic transaminase to evaluate the extent of improvement, if 1 determine histopathologically the effect of the treatmrrt on the granulomatous reaction and volume. Survival experiment was also performed to evaluate the effect of thiola treatment on the 1 i fe-span of bi 1 harzial mice pre-treated with praziquantel . The results so far obtained revealed that : Adninistration of either thiola or praziquantel to normal mice did not induce any significant changes in all the parameters studied. Bilharzial infection of mice produced significant abnormalities in the enzymatic activities ofthe liver tissue as manifested by the increase in alkaline phosphatase activity and the decrease in the activities of glucose-6-phosphatase,5’-nucleotidase, acetylchol inesterase, glutamic pyruvic transaminase and glutamic oxalacetic transaminase. The changes were much marked in long term infection than in short term infection. Similarly the total leucocytic count and the relative liver weight were markedly increased after bilharzial infection in both terms of infection, while total protein content of the liver di d not show any signi ficant change. Treatment of bilharzial infected mice with praziquantel produced significant increase in glucose-6-phosphatase,5’-nucleotidase, acetylcholinesterase and glutamic oxalacetic transaminase activities in case of short term infection and such improvements were less marked in long term infection and normal levels were not reached. While alkaline phosphatase,glutamic pyruvic transaminase and total proteins did not show any signi ficant change during the observation period in both terms of infection. On the other hand, total leucocytic count and relative liver weight were significantly reduced and reached the normal level after praziquantel treatmnt. Adninistration o f t h i o l a to bilharzial mice pre-treated with preziquantel caused further reduction in alkaline phosphatase activity and further increase in glucose-6-phosphate,5’-nucleotidase and glutamic oxalacetic transaminase activities. The effect o f t h - iola was much marked in short term infection than in long term irifect i on. Adninistration of thiola to bilharzial mice pre-treated with ntel produced substantial decrease in the mortality of infected mice. Treatment of bilharzial mice with praziquantel caused marked reduction in the granuloma volume in both terms of infection. Acininistration of thiola to bilharzial mice pre-treated with praziquantel caused further reduction in the granuloma volume.