الفهرس 
The reporter protein cd2Only 14 pages are availabe for public view
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Abstract
Molecular cloning provides a powerful way to investigate the
structure and function of surface proteins and to express them for large
scale protein production. Complementary DNA (cDNA) cloning strategies
can be used to analyse new membrane proteins. So far, the cloning of new
membrane proteins still depends on the rate at which monoclonal
antibodies are raised and analysed. Large number of monoclonal
antibodies have not been raised against the surface proteins of many
tissues.
Epitope insertion mutagenesis (EIM) has been devised to clone
cDNAs encoding membrane proteins in a way that should avoid the
existing need to develop antibodies against individual membrane proteins.
This is of particular importance when the suitable tissue is not available in
sufficient quantities to allow a sturation analysis of surface proteins
through the raising of monoclonal antibodies as in case of early embryonic
and extra embryonic tissues. In contrast, cDNA libraries can readily be
made from small quantities of starting tissues, can be propagated in
bacteriological cells and expressed at a relatively high level in mammalian
cells through a range of specialised expression vectors. Thus, a method for
screening cDNA libraries for sequences encoding membrane proteins
should greatly accelerate the rate of discovery of membrane proteins and
by directly yielding cDNA clones, would provide immediate access to the
structure of encoded polypeptide.