الفهرس 
INTRODUCTION AND AIM OF WORKOnly 14 pages are availabe for public view
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Abstract
This study aims to investigate the anti-cancer activity of nine legume seeds : chickpea, cowpea, faba bean, fenugreek, green pea, lentil, mung bean, soybean and white kidney bean. 300 gm of each legume were subjected to hydro-alcoholic extraction followed by a sequence of successive extractions using organic solvents. 500 gm from each legume were also used for extracting glycans. All extracts were tested for their cytotoxic activity on breast carcinoma cell line (MCF-7) in vitro. Samples showed a high cytotoxic activity on MCF-7 cells were studied for their cytotoxic activities on various cancer and normal cell lines in vitro. Also, polyphenols, saponins and alkaloids of fenugreek were studied for their cytotoxic activities on various cancer and normal cell lines and their effects on cisplatin’s cytotoxic activity on MCF-7 cells in vitro. Results revealed that the cytotoxic activity of fenugreek is due to compounds that are polar and get adsorbed on the surface of charcoal. Also, saponins of fenugreek increased the cisplatin’s cytotoxic activity on MCF-7 cells. This may be due to saponins increase the permeability of the cell membrane thus promoting the penetration of cisplatin into the cell. Chemical and phytochemical studies have been done on the studied legumes and their extracts. Also, their abilities on reducing nitrite were studied. Results revealed that tannins have a significant reducing effect, whereas glycans have a weak to moderate effect. Concerning fenugreek seeds, it was shown that fenugreek has an ability on reducing nitrite due to several components (alkaloids, tannins, saponins and glycans).