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Abstract Cotton is a major fiber crop grown throughout tropical and sub-tropical regions of the world. It also plays a principal role in the economy of a large number of developing as well as developed countries. Among the cotton growing countries, India ranks first in area (9.16 m.ha) and produces about 17.15 m bales of cotton Potdar I!I aL• 1999, from this 9.16 m.ha area 40% is hybrid cotton. The total hybrid seed requirement in India is 9000 tons annually Basu 1999; The commercial exploitation of the phenomenon of heterosis is one of the most important contributions from cotton breeding in this century. Heterosis is fundamentally dependent on the genetic effect of dominance ,Falconer 1960, • As a consequence, maximizing genetic diversity between inbreeds is a major goal in hybrid breeding programs in order to maximize heterozygosity in the progeny. Evaluation of hybrids for heterosis or combining ability in the field is expensive and time consuming. Though, many studies had been carried out attempting to predict heterosis and combining ability via biochemical genetic markers in a number of field crops to save time and field testing efforts. The use of molecular markers to identify quantitative trait loci affecting agriculturally important traits has become a key approach in plant genetics, both fur understanding the genetic basis of these traits and to design novel plant improvement programs. The main purpose of this study was performed to determine the amount of heterosis and combining ability for the important economic traits of cotton in intraspecific crosses of G. barbadel1se, L. In addition, to detect the possibility of predicting heterosis and combining ability in this crosses via biochemical analysis. |