الفهرس 
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Abstract
Lumpy skin disease (LSD) is an important infectious viral skin disease of cattle causing high economic losses .In the present study , a trial for isolation of LSDV from 76 samples (25 skin lesions, 12 nasal swabs, 12 heparinized blood and 18 internal organs samples) collected from clinically diseased and slaughtered cows via CAMs of ECEs aged 9 days, five egg passages were carried out for each sample. The isolated virus was identified using agar gel precipitation test (AGPT), latex agglutination test (LAT), reverse Passive haemagglutination test (RPHA), passive haemagglutination test (PHA) and neutralization test (NT). Detection of the LSDV in skin and different internal organs of infected cattle and in inoculated CAMs of ECEs using IF and IP techniques was carried out. Antigenic correlationship of skin isolate of LSDV, LSDV Ismailyia vaccinal strain, skin isolate of SPV and SPV Kenyan vaccinal strain using LSDV (Kenyan / 2490) strain and Kenyan SPV hyperimmune rabbit sera was studied. PCR was used for detection of the isolated virus in skin lesions and in inoculated CAMs. Also sequencing of amplified attachment gene skin isolate of LSDV, LSDV Ismailyia vaccinal strain, skin isolate of SPV and SPV Kenyan vaccinal strain. LSDV was isolated from collected samples. The isolated virus was identified using specific hyperimmune serum. Reverse Passive haemagglutination and latex agglutination tests are more sensitive for detection of LSDV than agar gel precipitation test. Indirect immunofluorescent and immunoperoxidase are quick and reliable methods for the virus identification. Field skin isolate of LSDV, LSDV Ismailyia88 vaccinal strain, field skin isolate of SPV and SPV Kenyan vaccinal strain are antigenically identical and can not differentiated by serological tests.Field skin LSDV isolate is genetically related to vaccinal LSDV (Ismailyia88 strain) than to vaccinal SPV (Kenyan strain) with reference to attachment gene sequence so use of specific vaccine prepared from LSDV for the disease control is essential instead of sheep poxvirus vaccine. Skin isolate of SPV may be LSDV that change to infect sheep. Isolated virus Can be named LSDV Mansoura 08.