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العنوان
Transplantation of isolated Islets of Langerhans fors the Treatment of Diabetic Rats /
المؤلف
Sobh, Mohamed Ahmed Mohamed.
هيئة الاعداد
باحث / Mohamed Ahmed Mohamed Sobh
مشرف / Mohamed A. Ghoneim
مناقش / Mohamed F. Ismail
مناقش / Mahmoud M. Gabr
الموضوع
Diabetes - Pathophysiology. Diabetes mellitus. Enzymes. Pancreas. Islands of Langerhans.
تاريخ النشر
1998.
عدد الصفحات
128 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الحيوان والطب البيطري
تاريخ الإجازة
1/1/1998
مكان الإجازة
جامعة المنصورة - كلية العلوم - Department of Zoology
الفهرس
Only 14 pages are availabe for public view

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Abstract

In this study isolation, purification and transplantatiojn of rat pancreatic islets were carried out for the treatment of diabetic rats. The recipient rats were made diabetic by a single intravenous injection of streptozotocin. Isolation of rat islets were done by injection of collagenase type XI through the bile duct. This procedure causes the complete destruction of pancreatic exocrine tissue, as well as mechanical separation and digestion of the interstitial tissue between the islets and the exocrine tissue, with the islets being preserved selectively intact. These islets were purified by ficoll density gradient and then hand picking under stereomicroscope.