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Abstract The partially purified soluble antigen of African horse sickness virus was separated from the virus particle and was concentrated up to loox of its original concentration. It was found that this antigen retained the whole antigenicity in the developed microslide technique of immunodiffusion test while the concentrated virus lost completely this antigenicity. The application of this technique using the partially purified soluble antigen revealed that the immunodiffusion reaction was group specific. Moreover, the precipitin antibodies started to appear as early as 3C days post inoculation and the clear distinct precipitin line was observed when the sera samples were used up to a period of 12 months post inoculation. The application of the microtechnique of complement fixation test using the partially purified soluble antigen of AHS. |