الفهرس 
General Inttoductiion.Only 14 pages are availabe for public view
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Abstract
The determination of trace components is emerging as a very important issue in the field of pharmaceutical analysis. It requires highly specific and accurate methods of analysis to be able to resolve the interferences that may arise during the analysis of these components. The present thesis deals with the term ”trace components” as a general term for analysis of the components with very low concentrations, such as biotin and those present as impurities during manufacturing like benzophenone in phenytoin dosage forms. Different analytical methods were developed for the determination of these compounds either in pure forms or in pharmaceutical preparations. Three derivative spectrophotometric methods, including zero-crossing first and third derivative and ratio derivative spectrophotometry, were developed for the analysis of benzophenone, as an impurity, in a hydantoin antiepileptic drug, phenytoin, bulk powder and its pharmaceutical preparations. A validated, simple and sensitive spectrofluorometric method was proposed for the determination of a water soluble vitamin belonging to the B-complex, biotin, in pure form and in certain pharmaceutical preparations. A kinetic spectrophotometric method was developed for the determination of biotin which based on its catalytic effect on the reaction between sodium azide and tri-iodide aqueous solution. The proposed procedure was successfully utilized in the determination of biotin in in certain pharmaceutical preparations. Biotin was also determined by a simple UV spectrophotometric method. The method is based on oxidation of biotin with excess potassium persulfate in acidic medium. In addition, a modified official HPLC method was constructed for resolving the peak of biotin from its oxidation product peak with a resolution factor of 4.44. The validation criteria of the developed methods were intensively studied. All the results were statistically analyzed and compared with those given by official or comparison methods.