الفهرس 
Growth habit and advantage of Sour orange and Volkamer lemon citrusOnly 14 pages are availabe for public view
 |  | from | 243 |  |  |
| | | from | 243 | | |
Abstract
The present investigation was carried out during the period from 2004 to 2007 in tissue culture laboratory and greenhouse of Horticulture Department, Agriculture Faculty, The University of Mansoura, Egypt. It was aimed to study the potential uses of tissue culture techniques for rapid and economical in vitro micropropagation of Citrus rootstocks namely, Sour orange (Citrus aurantium, L.) and Volkamer lemon (Citrus Volkameriana, Ten.). Micrografting technique was done onto two citrus scions onto two citrus rootstock. In the same time, the resulted rootstock plantlets were used for in vitro micrografting processes. Factors considered in this research to activate efficiency of plantlets regeneration from the cultured explant tissues were explant types (Shoot tips and Epicotyls) and media supplemented with either 2 growth regulators are known to be important for shoot proliferation namely, 6-benzylaminopurine (BAP) at 0.00, 0.25, 0.50, 0.75, 1.00, 1.25 and 1.50 mg/L; Kinetin (Kin) at 0.25, 0.50 and 1.00 mg/L added solely or in combinations along with media containing 2 auxins had a positive effect on root proliferation namely, Indole-3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA) added either solely at 0.00, 1.00, 2.00, 3.00 and 4.00 mg/L each or in combinations at 0.50, 1.00, 1.50 and 2.00 mg/L each. The former media also combined with a fixed 0.50 mg NAA/L, whereas a fixed 0.50 mg Kin/L added to the later ones.
* This study revealed the following results :-
1- Epicotyls are the preferred explants to in vitro propagation of Sour orange and Volkamer lemon Citrus rootstocks.
2- The concentration of BAP in complete formation medium for shoot proliferation is considered the limited factor affecting characteristics of the proliferated shoots on both explants type. The superiority was to BAP at 1.50 mg/L. It was the best to produce significantly the highest leaves and shoots number per explant.
3- The concerned results of the tested shoot proliferation media in complete formation pointed to medium consisted of solid MS (1962) basal medium at full strength supplemented with BAP at 1.50 mg/L and kin at 1.00 mg/L plus a fixed 0.50 mg NAA/L is uniquely suited to obtain the best shoot proliferation on both explants type of both Citrus rootstocks used.
4- Data of micrografting indicated that grafting Washington navel onto sour orange citrus rootstock was better than Valencia onto the same rootstock.
5- Also the data showed that grafting Valencia onto volkamer lemon citrus rootstock was better than Washington navel onto the same rootstock.