الفهرس 
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Abstract
The present study was carried out at Horticulture Department, Faculty of Agriculture, Mansoura University throughout the period from 2005 to 2008 for two parts of the experimental work. The first part was in vitro culture to study the effect of explant types, shoot tip and nodal cuttings with 1,2 and 3 axillary buds and different growth regulators (BA, Kin, AS, BG) on micropropagation of Aster novi-belgii . Murashige and Skoog (1962) medium was used and supplemented with sucrose (30g/l) and 7g/l Agar agar. The obtained results showed that benzyleadenine (BA) at 1 ppm was significantly effective in enhancing the number of shoots per explant and induced more callus formation on the base of shoot tip explant than the other used growth regulators, i.e, kinetin (Kin), adenine sulphate (AS) and phloroglucinol (PG). Concerning the response of nodal explant type to growth regulators, the data indicated that the explants have 2 or 3 nodes were significantly effective in enhancing the number of shoots / explant, shoot length, leaves number per shoot and callus formation. Also, the highest response of shoot number / explant was recorded by BA. The effect of interaction between nodes number / explant and growth regulators showed that the highest number of shoots / explant was obtained with the combination of three nodes / explant and BA at 1 ppm, while Kin at 1 ppm combined with three nodes / explant was the most effective in enhancing roots number / shoot. Well rooted shoots were acclimatized successfully on a media of (1:1 , v/v) Peat moss and sand to obtain good seedling of Aster novi-belgii. The second part of the work aimed to study the effect of some preservative treatments on post harvest characters of gladiolus cut flower spikes (Gladiolus hyprida cv. Rose Supreme). The pulsing solution treatment used was siliver thiosulphate (STS) for 30 minutes. whereas, the holding solutions used were sucrose (S) at 10% in the first season and 5% in the second one, citric acid (CA) at 150 ppm, 8-hydroxy quinoline (8-HQS) at 200 ppm and yeast extract (Y) at 1mg/ L either alone or in combinations. the obtained results showed that using yeast extract alone as a preservative solution in vase after pulsing the spikes with silver thiosulphate (STS) for 30 minutes, showed a promising effect on enhancing the studied parameters of gladiolus cut flower spikes as florets weight, spike fresh weight, opening percentage, wilting percentage, absorbed solution and flower longevity as well as the other preservative solutions. So, under the condition of this study this treatment could be recommended as a holding solution for gladiolus cut flower spikes as a safe, cheap and available solution