الفهرس 
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Abstract
Introduction : The present study was carried out during the years 2003 and 2004 on 250 non pregnant healthy buffalo-cow aged from 58 years in the abattoir of Mansoura city, Dakahlia province Egypt . The reproductive tract was removed shortly after evisceration . The vagina was tied externally to avoid any contamination during transport . The organs were placed in a sterile container and taken immediately to the laboratory. Material and Methods: The general picture of the organs were described. The phase of the estrous cycle was determined depending on the ovarian structures. Genitalia were classified into organs in follicular phase, luteal phase, organs with inactive ovaries and with cystic ovarian degeneration. Any gross abnormalities in the genitalia were reported. Transrectal scanning of the animals were carried out with Ultrascan 50 which provided with transrectal transducer (5 MHz). For histopathological examination, a section of about 1x1 cm was taken from the uterine horn, prepared and stained with H & E stain. Swabs were taken from the uterus after being sterilized with a hot spatula and cut by sterile scissors. Swabs were immersed in nutrient broth and incubated at 37 <U+00BA>C overnight. The cultures were streaked on nutrient agar, 10% sheep blood agar and MacConkey?s agar. Plates were incubated at 37 c for 2472 hrs. Colonies were purified and identified. Results: Naked eye examination revealed that, 36.40% or genitalia were in follicular phase, 23.60% in luteal phase, 34.00% with inactive ovaries and 3.60 % with cystic ovarian degeneration. Also from the abnormalities there were 2.40 % pyometra 1.6% with paraovarian cyst and 12.00 % were affected with endometritis. Out of 181 normal uteri, 18 organs proved to be bacteria free. from 173 organs, 215 bacterial isolates were obtained. The most predominant isolates were The predominant isolates in a descending order were : Staphylococcus epidermidis (24), Escherichia coli (25), Streptococcus faecalis (20), Gaffkya tetragona (18), Micrococcus luteus (17), Streptococcus ubris (17), Sarcina lutea (12), Corynebacterium pyogenes (11), Corynebacterium bovis (12), Pseudomonas aeruginosa (11), Corynebacterium ovis (11), Corynebacterium renale (9), Proteus vulgaris (9), Streptococcus durans (8) and Corynebacterium equi (6). Out of 34 organs with mild degree endometritis, 36 isolates were obtained. The most prevalent bacterial isolates were Corynebacterium pyogenes (5), Micrococcus luteus (4) Staphylococcus aureus (4), Staphylococcus epidermidis (3), Anthracoids (3), Corynebacterium ovis (3), Sarcina lutea (2), Streptococcus pyogenes (2), Streptococcus durans (2), E. Coli(2) Gaffkya tetragona (1), Streptococcus ubris (1), Streptococcus faecalis (1), Corynebacterium bovis (1), Corynebacterium equi (1), Pseudomonas aeruginosa (1) .