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Abstract The present study aimed to investigate the response of three sunflower (Helianthus annus, L.) genotypes (vidoc, Euroflour and Miak) to some biofertilization treatments and nitrogen fertilizer levels in pot and field experiments. Experiments were carried out at the Experimental Farm, Faculty of Agriculture, Mansoura University. 1. Pot Experiment: The pot experiment was carried out in the greenhouse for 21 days at the Department of plant pathology, Faculty of agriculture, Mansoura University during the summer of season 2000 to evaluate the response of three sunflower genotypes to some biofertilization treatments under sterilized soil and unsterilized soil conditions. A factorial experiment in complete randomized design with four replicates was used. The combination of the three studied factors resulted 72 pots in this experiment. The first factor was occupied with the following two different soil conditions: 1 Sterilized soil. 2 Unsterilized soil. While the second factor was devoted to the following three sunflower genotypes: 1 vidoc: French hybrid. 2 Euroflour: French hybrid. 3 Miak: Russian open pollinated cultivar. The third factor was assigned to the following three biofertilization treatments: 1 Uninoculation (without biofertilization). 2 Inoculation with the mixture of A.b (No. 40) and A.l (Br17). 3 Inoculation with the mixture of B.p (B32) and B.p (CF43). The field experiments aimed to study the effect of biofertilization and nitrogen fertilizer levels on growth, yield and yield components as well as quality of sunflower genotypes (Helianthus annus, L.) Vidoc, Euroflour and Miak. Also, analytical electrophoresis of protein seeds. Three field experiments were carried out at the Experimental Station, Faculty of agriculture, Mansoura University during the three successive growing seasons of 2000, 2001 and 2002. A stripsplit plot design with three replicates was used. The horizontal strips were randomly assigned to the following the three same sunflower genotypes which used in the pot experiment. While, the main vertical strips were occupied with the following three biofertilization treatments: 1 Uninoculation (without biofertilization). 2 Inoculation with A.b (No. 40) and A.l (Br17). 3 Inoculation with B.p (B32) and B.p (CF43). The sub vertical plots were devoted to the following three nitrogen fertilizer levels: 1 15 kg N/fad. 2 22.5 kg N/fad. 3 30.0 kg N/fad. from the previous results, it can be concluded that planting inoculated seeds with Azospirillum brasilense and Azospirillum lipoferum of the genotype Euroflour and adding 30.0 kg N/fad will achieve high productivity and good quality of sunflower under Mansoura district, Dakahlia Governorate. |