الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Gastroenteritis is an important cause of morbidity and mortality in children as well as adults. Although the causative agents depend on the patients’ age, predisposing conditions and geographical area, non-typhoidal Salmonellae are important food-borne pathogens that cause gastroenteritis. Over the period of this study, out of 420 adult patients complaint of gastroenteritis in Main Hospital and Emergency Hospital, non-typhoidal Salmonellae strains were isolated from 18 cases (4.28%) and out of 150 patients complaint of gastroenteritis in Mansoura Children Hospital, non-typhoidal Salmonellae strains were isolated from 4 cases (2.66%). From 80 food handlers studied in MUHs, non-typhoidal Salmonellae strains were isolated from 3 cases (3.75 %). The recovery of non-typhoidal Salmonellae strains was higher (100%) after enrichment in selenite F broth than by direct plating (84%). Non-typhoidal Salmonella gastroenteritis was more common in outpatients, age group < 20-49 years and warmer months. The incidence of non-typhoidal Salmonella was higher in asymptomatic food handlers than symptomatic food handlers. Salmonella enteritidis was the most prevalent serotype (52%), followed by Salmonella typhimurium (40%) and finally Salmonella virchow (8%). High resistance to ampicillin, chloramphenicol tetracycline and trimethoprim-sulphamethoxazole are detected among the three serotypes. Most of ampicillin resistant non-typhoidal Salmonellae (92%) produced ?- lactamase. Imipenem is the most effective antibiotic for treatment of non-typhoidal Salmonella gastroenteritis. Resistance to gentamicin and ciprofloxacin is accounted for 20% and 4%, respectively This work was carried out to clarify the role of non-typhoidal Salmonellae as causative agents of gastroenteritis in Mansoura University Hospitals (MUHs), with special focusing on their phenotypic characters, serotyping, antibiotic resistance and p¬lactamase production. The current study extend over period of 15 months, from March 2001 until May 2002 and involved collection of stool specimens from 420 adult patients admitted to or attended Mansoura University Hospitals (MUHs) Wards and Outpatient Clinics with suspected gastroenteritis, 150 patients complaint of gastroenteritis at Mansoura Children Hospital and 80 gastroenteric symptomatic and asymptomatic food handlers (30 and 50 stool samples, respectively) working at MUHs. Fifty stool specimens were collected from healthy persons as control group. Twenty-five non-typhoidal Salmonellae (NTS) strains were isolated from 650 stool specimens and no strain was isolated from the control group. Non-typhoidal Salmonellae were identified using colonial morphology and Gram-stained smears after culturing stool on XLD agar, SS agar and MacConkey agar before and after selenite F broth enrichment where the recovery of non-typhoidal Salmonellae strains .