الفهرس 
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Abstract
The present work investigates the effect of large doses of epinephrine and .i norepinephrine on the release of myocardial isoenzyme Creatine kinase - MB CK _ MB) as an index of myocardial damage both in vitro and in vivo, in rabbits. Also to test the possible cardioprotective role of propranolol, phentolamine and erapamil as a beta adrenergic blocker, an alpha adrenergic blocker and a Ca - ,•.hannel blocker, respectively. he experiments were performed in two parts. fA) In vitro study : in which the heart was isolated and perfused according to :Langendorff technique, using Ringer-Locke solution, well oxygenated and ifemperature kept constant at 36Co- 39Co . The drugs tested, were injected through the rubber cannula. The perfusate issuing from the heart was collected for determination of CK-MB activity level . Experiments include tile following groups: 1- Effect of 10 -6M of epinephrine and of norepinephrine on the .level of CK- MB in the perfusate at 10, 15,20 and 30 minutes after addition of the drugs. 2- Effect of beta adrenergic blocker : propranolol, added 10 minutes pre and post epinephrine & norepinephrine, on the level of CK - MB in the perfusate collected 20 minutes post epinephrine & norepinephrine. 3- Effect of alpha adrenergic blocker : phentolamine added 10 minutes pre and post epinephrine & norepinephrine, on the level of CK-MB in the perfusate collected 20 minutes post epinephrine & norepinephrine 4- Effect of Ca channel blocker : verapamil , added 10 minutes pre and post epinephrine & norpinephrine on the level of CK - MB in the perfusate collected 20 minutes post epinephrine & norepinephrine.