الفهرس 
PolymorphismOnly 14 pages are availabe for public view
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Abstract
Periodontitis is an inflammatory destruction of periodontal tissue with combined genetic and environmental etiology. Immune regulatory dysfunction of cytokine function and release is postulated to affect both host response and clinical behavior This work organized to check for the association of genetic polymorphisms of cytokine genes with the susceptibility and /or severity of Periodontitis in Egyptian cases from the Nile Delta Region. This casecontrolled crosssectional study included 46 cases with periodontitis in addition to 98 healthy unrelated controls. The cases were recruited from the Department of Oral Medicine and Periodontology, Mansoura University, Egypt. Diagnosis and classification of disease severity were made on the basis of history and clinical examination. Cases were classified into aggressive or early onset periodontitis (36.9%) and chronic or adult periodontitis (63.04%). Furthermore, chronic cases were sub classified into moderate (72.41%) and severe (27.6%) disease. For all cases and controls, DNA was extracted and amplified using polymerase chain reaction with sequence specific primers (PCRSSP) for detection of single nucleotide polymorphisms (SNPs) in the promoter regions of cytokine genes, TNFgas308 (G/A), IL101082(G/A), IL6174(G/C) as well as IL1RaVNTR in intron 2 of the gene. Total cases showed high significant frequency of homozygous IL101082(A/A) (OR=3.97, P<0.05), TNFgas308(G/G) (OR=6.71, P<0.001), IL6174 C/C (OR=8.18, P<0.001) and IL1Ra (A1/A1) (OR=3.42, P<0.05) genotypes. These were considered risk genotypes for disease susceptibility. The same was shown by cases with chronic periodontitis while cases with aggressive periodontitis showed only high significant frequency of homozygous IL6174(C/C) (OR=8.36, P<0.05) and TNFgas308(G/G) (OR=4.72, P<0.05) genotypes. Cases with severe disease showed high significant frequency of homozygous IL1Ra (A1/A1 ) (OR=12.28, P<0.05) genotype. On the other hand, combined heterozygosity genotypes including IL101082 (G/A) and/or TNFgas308 (G/A) (OR=0.29, P<0.001) showed lowest significant frequency among all cases were considered low risk genotypes. Cytokine gene polymorphisms may be used as a marker for periodontitis susceptibility, clinical behavior and severity helping for induction of prophylaxis to young members of affected family against disease evolution and progression.