الفهرس 
Materials and methodsOnly 14 pages are availabe for public view
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Abstract
Many biosurfactant producers and hydrocarbon degrading bacteria were isolated from Egyptian Mediterranean sea shore. Most active isolates identified as Pseudomonas aeruginosa, Flavobacterium brave, F. odoratum, Moraxella osloensis, B. cereus, B. thuringiensis and B. subtilis. The higher biosurfactant producers were optimized to increase their productivity. Biosurfactants were characterized according to their stability under extreme conditions of temperature, salinity, pH, and presence of heavy metal. They are relatively highly stable in most cases. The effectiveness of the studied isolates in degradation and remediation of diesel oil appears absence of about 50% or more of a substrate after 4 days. The chemical structure of biosurfactants investigated usually rhamnolipids and/or fatty acids. Large part of rhlAB operon of Pseudomonas aeruginosa was isolated by amplification technique to produce 2.3Kb DNA fragment. The PCR product cloned and characterized by restriction digestion to confirm with known sequence. The fragment is closely related with known sequence except HidIII one site. The result can be used to confirm identification of local isolate. The PCR clone was induced to transcription and translation of it protein. The produced protein appears at target protein size 32KDa and 30 KDa. Key words: 1 Biodegradation. 2 Biosurfactant . 3 Rhamnolipid gene