الفهرس 
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Abstract
ٍS. marcecsens, a gram-negative bacterium belonging to the family Enterobateriaceae, is one of the most efficient bacteria for degradation of chitin. Production of chitinolytic enzymes in S. marcescens is induced by the presence of chitin in the culture medium. The major objective of this dissertation was the cloning of chitinase from a local isolate S. marcescens to be used later in the production of transgenic plants. The ultimate goal of this work was to introduce the chitinase gene into plants or rhizosphere bacteria to improve their ability to control phytopathogenic fungi, therefore, can substitute the use of fungicides as classical control agents of fungal infection.