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Abstract Background: Cancer is a major public health problem worldwide (in both developed and developing countries) and is the second leading cause of death Cancer is the disease characterized by uncontrolled and unregulated proliferation of the cells. Aim: evaluation the chemically characterize of bee pollen, using NMR and LCMS tools, identify the bioactive compound (s), elucidate their structure as part of our ongoing project on anti cancer activity on in vivo Ehrlich tumor model. Methods: Female Swiss mice weighing 24 ± 4 g (6–8 weeks old) were supplied by bee pollen from the National Research Center, Giza, Egypt. All animals were kept in a standard laboratory setting with a 12-h light/dark cycle. The rodent food (El-Haramain rodent diet, Egypt) and water were supplied. Acclimatization to laboratory conditions for 12 days prior to the experiments was done to all mice. Results: Phytochemical analysis of bee pollen indicated the presence of various bio active compounds such as flavonoids, glycerophospholipids, Phenolic acids, dialkylamines. Flavonoid such as isorhamnetin-3-O-triglucoside, Quercetin, Isorhamnetin 3-O-hexoside, Isorhamnetin 3,7- di-O-hexoside, glycerophospholipids such as 1-(1Z-Hexadecenyl)-sn-glycero-3-phosphocholine and 1-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phosphocholine. Amino acids such as N 1 , N 5 , N 10 -tricaffeoylspermidine. Previous studies on bee pollen found N1, N10-di-p-coumaroylN5-caffeoyl spermidine, N1, N5-di-p-coumaroyl-N10- hydroxyferuloyl spermidine, N1, N5-di-pcoumaroyl- N10-feruloyl Spermidine, N1-p-coumaroyl-N5, N10-di-caffeoyl spermidine. Oral administration of 300 and 600 mg\ kg suggest has antitumor Ehrlich effect on ascites carcinoma 13 in vivo. The mechanism underlying its antitumor activity may be attributed to its antioxidant properties |