الفهرس 
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Abstract
The present study was carried out at commercial hatchery lab in private
farm. The experiment was extended from 4 November 2020 to the end of
January 2021, in order to investigate the effect of in ovo feeding and early
access of diet in the hatcher on the physiological performance and immunity of
broiler chicks.
The aim of this study was to evaluate the effect of in ovo injection with
different ratios (1,3g) into cobb 500 eggs at different embryonic developmental
stages and on the survival, hatchability and body weight from post-hatch to 35
days (the final BW).
This experiment was performed using 540 fertile commercial eggs of
Cobb 500. All eggs were obtained from the same breeder flock and were laid
within a 24-hour period. Humidity and temperature during first 18 days of
incubation were set at 60% and 37.8°C, respectively. On 18.5 day of incubation
all eggs were in ovo-injected into the air cell by using an automatic needle, and
0.5 mL of the L-arginine solutions was injected in a depth of about 15 mm.
These eggs were randomly allocated to six treatment groups were;
A negative control group ” 110 fertile eggs ” (un-injected; T1).
A positive control group ” 110 fertile eggs ” (injected with sterile
distilled water (T2).
Injected with 1% L-arginine ” 80 fertile eggs ” (1 g L-arginine /100 ml
sterile distilled water and have been added 500-gram starter feed in the
hatcher; T3).