الفهرس 
AcknowledgmentOnly 14 pages are availabe for public view
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Abstract
This research presents a validated, multipurpose and sustainable HPLC-DAD
method intended for the separation and simultaneous determination of three related
non-steroidal ant-inflammatory drugs (NSAIDs): diclofenac sodium, bromfenac
sodium and nepafenac in the presence of five of their related compounds and
suspected impurities. A Venusil XBP Cyano column with gradient elution of a
mobile phase involving methanol, acetonitrile and mixed phosphate and sodium
pentane sulfonate buffer (pH 2.5) were employed. DAD was tuned at 210, 233 and
275 nm for measuring peak areas of different analytes. The proposed procedure was
successfully validated and utilized in comprehensive stability study of the
investigated drugs. The three NSAIDs have been determined within a linearity range
of 5–150 µg/mL with excellent analytical performance. The method targeted their
analysis in variable dosage forms particularly ophthalmic liquid preparations. This
has evolved the need to determine benzalkonium chloride as well which is a common
preservative in such dosage forms. It was measured in the range 10–200 µg/mL. The
least detectable concentration levels of the analytes were within the range 0.5–2.0
µg/mL. Comparing the designed method to a number of reported ones with respect
to greenness and whiteness/sustainability merits using AGREE and RGB-12
paradigms proved it is significantly eco-friendly and represents a green/white option
for multi-analyte stability indicating analysis of the above-mentioned drugs.