الفهرس 
INTRODUCTION AND AIM OF WORKيوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
Cisplatin is a potent platinum anticancer drug that has a great ability to treat various types of solid tumors such as breast, ovarian, testicular, head and neck, and uterine cervical carcinoma. Previous results showed that chronic administration of cisplatin can lead to oxidative stress, cytotoxicity, DNA damage, and organ toxicity.Bilberry (Vaccinium myrtillus L.) is a deciduous shrub in the Ericaceae family, which grows mostly in Northern and Central Europe known as European blueberry. It is a rich source of antioxidants and is currently being investigated for its antioxidant properties, and capillary vessel protection it is used for improving sight including night vision with the treatment of ocular disorders. Aim of the work The present study was designed to examine the possible protective effect of Vaccinium myrtillus L extract against testicular toxicity induced by cisplatin in male rats. This study was achieved by using male Wistar albino rats, 7-8 weeks old, with an average weight of 210g. Animal grouping The animals were divided into four groups (10 rats /group) as follows: 1. Control group: received a single dose of 0.9% saline orally of volume (1.0 ml). 2. Bilberry group: rats received bilberry treatment given orally in a dose of 200 mg/kg b.w for 10 days. 3. Cisplatin group: a single dose of 7.5 mg/kg b.w of cisplatin were administered intraperitoneal (IP).4. Bilberry and cisplatin group: 7.5 mg/kg b.w of cisplatin were administered IP, and 200 mg/kg b.w of bilberry were given orally by stomach tube. Bilberry treatment were applied for 10 successive days starting 1 day before cisplatin administration. Results At the end of the experimental period (10 days) blood and semen samples were collected for different parameters. The obtained results can be summarized as follows: A-Cisplatin administration 1- Semen changes:•A significant decrease in testes’ weight•A significant decrease in sperm count•A significant decrease in sperm motility•A significant decrease in viability• A significant decrease in normal sperms•A significant increase in abnormal sperm 2-Biochemical changes•A significant increase in serum MDA content •A significant increase in serum H2O2 content•A significant decrease in serum CAT activity•A significant decrease in serum SOD activity•A significant decrease in serum GSH activity•A significant decrease in serum GST activity•A significant decrease in serum FSH level•A significant decrease in serum LH level•A significant decrease in serum testosterone level3-ApoptosisA significant increase in apoptosis •A significant increase in serum p53 level•A significant increase in serum cytochrome c levelA significant increase in serum caspase 3 level•A significant increase in serum caspase 9 level•A significant increase in serum Bax level•A significant decrease in serum bcl2 level4-DNA comet assayA significant increase in DNA damage where cells with fragmented DNA appeared as distinct heads with tails.5-Histopathological observations CP administration showed considerable number of damaged testicular cells that have lost their characteristic appearance, with degenerated spermatids with arrest of spermatogenesis at 2ry spermatids. B- Bilberry extract administration with cisplatin The obtained data showed that administration of bilberry extract with cisplatin caused a marked improvement in most biochemical and histopathological investigations induced by cisplatin Conclusion and recommendations The present results indicated that bilberry extract acts as a good protective agent against testicular disorder and oxidative stress associated with cisplatin. This may be attributed to its ability to scavenge reactive oxygen species that are responsible for testicular injury. Moreover, bilberry extract administration before and during cisplatin administration may help in the safe application of testicular injury, however, we recommended that bilberry extract could help in the development of ideal protection for the testis in the future.