الفهرس 
Antimicrobial Drug ResistanceOnly 14 pages are availabe for public view
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Abstract
Background: Antimicrobial resistance is now considered a major crisis to human health in 21st century. Polymixins were ignored for many years because of their toxicity. Now are considered last treatment option for serious infections caused by multidrug resistant gram negative bacteria
(MDR-GNB).
Objectives: The aim of this study is to determine the prevalence of colistin resistance among gram negative bacilli isolated from patients admitted at Fayoum University Hospital and to compare colistin resistance among different gram negative bacilli spp.
Methods: This observational cross- sectional study was conducted over the period from January to June 2023. A total of 115 isolates of MDR-GNB were obtained from patients admitted to different departments of Fayoum University Hospitals. Full identification was done by conventional microbiological methods, Then all isolates were subjected to broth microdilution for determination of Minimum Inhibitory Concentration (MIC) & MacConkey Colistin agar for detection of colistin resistance.
Results: The prevalence of colistin resistance by MIC among MDR-GNB was found to be 12.2% (14 out of 115) as follows: 28.6% among Ecoli, 35.7% among Klebsiella spp, 28.6% among Pseudomonas spp and 7.1% among Acinetobacter spp while the prevalence of resistance by MacConkey colistin agar was found to be 38.2% (44 out of 115) as follows: 40% among Ecoli, 34% among Klebsiella spp, 42.3% among Pseudomonas spp and 40% among Acientobacter spp with accuracy 88.3%, specificity 81.3% and sensitivity 97.6%.
Conclusion: The use of MacConkey Colistin agar is an easy method for screening of colistin resistance which is currently a major concern. However, Broth Micro Dilution (BMD) is considered the gold standard.
Key words: Polymixins, Colistin resistance, Broth Micro Dilution.