الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 142 |  |  |
| | | from | 142 | | |
Abstract
Scientific attention has been drawn to silver nanoparticles, since silver
ions have long been recognized as antimicrobial agents. Silver
nanoparticles (Ag-NPs) have a unique mode of action as antibacterial
agents in addition to their anticancer and antioxidant properties. A
microbial nanotechnology approach was used in this study to synthesize
Ag-NPs using a cell filtrate of actinomycete isolate that isolated from soil
at Shebin el kom, Menoufia, Egypt. In a screening process for the
biosynthesis of silver nanoparticles (Ag-NPs), it was discovered that only
one of the 13 actinomycetes that were investigated can produce Ag-NPs.
Ultraviolet-visible (UV-Vis), Fourier transform infrared (FT-IR), X-ray
diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), scanning
electron microscopy (SEM), and transmission electron microscopy (TEM)
were the techniques that utilized to validate the synthesized silver
nanoparticles (Ag-NPs). Additionally, the optimal conditions for synthesis
of Ag-NPs were tested depending on various parameters. Moreover, the
anticancer, antibacterial, and antibiofilm activities of silver nanoparticles
were investigated. It was established by the X-ray diffraction (XRD)
examination that the crystal structure under consideration is a face-
centered cubic (FCC) pattern and that the sample was crystalline in nature.
TEM analysis revealed that the Ag-NPs that were produced with the Ag-
NPs have a spherical shape and an average size of 32.2 nanometers.
Moreover, Fourier transform infrared spectroscopy (FTIR) indicated
considerable changes in functionality following the dispersion of Ag-NPs.
These changes could be attributable to the ability of the cell filtrate of
Streptomyces enissocaesilis BS1 to behave as both a reducing agent and a
capping agent. The synthesized Ag-NPs had exceptional antibacterial
activity against a variety of pathogenic strains.