الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
The amniotic membrane (AM) holds significant promise in regenerative medicine due to its unique properties, including low immunogenicity and rich content of growth factors, cytokines, and extracellular matrix components. These qualities make it valuable for applications such as ocular surface reconstruction and wound healing. The accessibility of the AM during childbirth enhances its convenience and ethical use, making it a promising tool for addressing a wide range of medical conditions.
While the use of intact or preserved AM as an allograft is well-established, recent research focuses on enhancing its properties through modifications such as creating amniotic membrane extract (AME). This approach simplifies the therapeutic process and eliminates surgical risks, offering a convenient alternative. The exploration of such modifications reflects a growing interest in optimizing AM characteristics for regenerative medicine applications, highlighting the need for standardized preparation methods to ensure consistent therapeutic outcomes.
Anatomically, the AM can be categorized into placental, reflected, and umbilical membranes, with each region exhibiting distinct biological properties. Regional variations within the AM, including differences between placental and reflected regions, influence the AME composition, and may dictate its suitability for specific therapeutic applications. selecting the appropriate AM sub-region based on its composition is essential for maximizing therapeutic efficacy.
The aim of the study was to assess the total protein content and concentrations of HGF and TGF-β in amniotic membrane extract (AME). Additionally, we sought to compare the concentrations of these growth factors between the placental and reflected regions of the amniotic membrane (AM). Another objective was to optimize the method for preparing AME to enhance its efficacy for potential therapeutic applications. The study involved 40 amniotic membranes harvested after elective caesarean section, with each membrane divided into reflected and placental amnion segments. Measurements of total protein, HGF, and TGF-β were conducted for all 80 samples to enable a comprehensive comparison between the two regions.
The study compared total protein content and concentrations of TGF-β and HGF between reflected and placental amniotic membrane regions. While no significant difference in total protein content was observed, TGF-β levels were significantly higher in the reflected amnion, and HGF levels were higher in the placental region. Additionally, a weak positive correlation was found between total protein content and levels of HGF and TGF-β in both amniotic membrane extracts