الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 106 |  |  |
| | | from | 106 | | |
Abstract
Maximising the effectiveness of cardiac revascularization treatment requires early detection of acute myocardial infarction (AMI), one of the leading causes of disease and mortality. In order to avoid myocardial ischemia and the cellular death that follows, new biomarkers are needed to shorten the time it takes for MI to be diagnosed and for therapeutic measures to begin.
In the pathophysiology of cardiovascular illnesses, microRNAs and long non-coding RNAs have recently gained interesting diagnostic and prognostic markers. The stability of lncRNA and miRNAs in plasma, serum, and urine, together with their cell-specific physiological roles, make them promising new biomarkers for AMI.
The study’s objective is: The purpose of this study was to investigate the relationship between the start of acute myocardial infarction and the expression pattern of lncRNA ANRIL and miRNA-125a. The study’s secondary objective was to investigate the role of miRNA-125a and lncRNA ANRIL expression in diagnostics.
Standard practices: Fifty patients with acute myocardial infarction (AMI) from Minia University Hospital’s critical care unit (CCU) and fifty healthy volunteers made up the current study. Every patient had 6 millilitres of their venous blood sampled. We used real-time PCR to assess serum ANRIL and miRNA-125a. After isolating total RNA with preserved lncRNAs from 200 µL of serum from every patient, we collected clinical data.
Importantly, when comparing the groups with and without acute MI, we found that miRNA-125a expression levels were significantly higher in the former (P = 0.001). In contrast, the acute MI group’s ANRIL expression levels
were significantly lower than the control group’s (P = 0.001). Additionally, we found that miRNA-125a had an 83.3% sensitivity and an 81.2% specificity for predicting left ventricular failure in AMI patients with LV dysfunction, with a substantial increase in expressions (P value <0.001). Additionally, ANRIL demonstrates a reduction in expressions in AMI patients with LV dysfunction (p
<0.001), and it has a sensitivity of 94.4% and specificity of 75% in predicting the presence of left ventricular dysfunction.
Conclusion:
• The current study reported significance of miRNA-125a and ANRIL as a potential marker in diagnosis of acute myocardial infarction.
• The present study highlighted the important role of miRNA-125a and ANRIL as potential diagnostic marker with LV dysfunction.